Rennie R J, Schmidt E L
Can J Microbiol. 1977 Aug;23(8):1011-7. doi: 10.1139/m77-150.
Certain steps of a protocol to enumerate a bacterium directly in soil by immunofluorescence were studied with respect to the enumeration of Nitrobacter in soils of diverse properties. Maximal counts of Nitrobacter were obtained by varying factors involved in the release of bacteria from the soil. Differences with respect to these factors were related to soil-colloidal properties. Enumeration protocols modified with regard to soil properties were used with strain-specific fluorescent antibodies (FA) to enumerate Nitrobacter populations (a) in soil during storage, (b) in comparison with most probable number (MPN) enumeration, and (c) occurring in a spectrum of soil samples. FA counting was rapid and precise and gave counts generally 10- to 100-fold higher than obtained by MPN. Nitrobacter cells of the two serotypes studied, designated as agilis and Winogradsky, occurred in each soil at levels of 10(4)-10(5) per gram.
针对通过免疫荧光法直接在土壤中计数细菌的某一方案的特定步骤，研究了其在不同性质土壤中硝化细菌计数方面的情况。通过改变土壤中细菌释放所涉及的因素获得了硝化细菌的最大计数。这些因素方面的差异与土壤胶体性质有关。针对土壤性质进行修改的计数方案与菌株特异性荧光抗体（FA）一起用于（a）在储存期间的土壤中、（b）与最大可能数（MPN）计数相比较以及（c）在一系列土壤样品中计数硝化细菌群体。FA计数快速且精确，所得计数通常比MPN法高10至100倍。所研究的两种血清型的硝化细菌细胞，分别命名为敏捷硝化细菌和维诺格拉德斯基硝化细菌，在每种土壤中的含量为每克10⁴ - 10⁵个。
