Liu Shanshan, Guo Lining, Chen Min, Liu Wei, Li Yanhong, Wang Xiaoxu, Li Shilan, Zhang Jie, Ni Xin
Department of Otorhinolaryngology Head and Neck Surgery, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing, China.
Beijing Key Laboratory for Pediatric Diseases of Otolaryngology Head and Neck Surgery, Beijing, China.
Front Pediatr. 2021 Nov 5;9:732973. doi: 10.3389/fped.2021.732973. eCollection 2021.
The present study aimed to assess the expression of caspase-1 and caspase-1-dependent processing of cytokines, such as interleukin (IL)-1β and IL-18, in the middle ear effusion of children with otitis media with effusion (OME) in order to identify the potential role of inflammasomes in OME. This study included 29 children scheduled for myringotomy with the insertion of tympanostomy tubes due to OME. Middle ear effusion (MEE) was collected during the surgery. Caspase-1, IL-1β, and IL-18 were assayed using enzyme-linked immunosorbent assay kits. The levels were compared between those with mucoid and serous MEE and those with and without a history of ventilation tube insertion. Caspase-1, IL-1β, and IL-18 were detected in all samples. The caspase-1, IL-1β, and IL-18 levels did not significantly differ between mucoid samples and serous samples. No statistical significances were discovered in caspase-1, IL-1β, and IL-18 levels between with and without a history of ventilation tube groups. There was a significant negative correlation between IL-1β and IL-18 and the duration of OME ( < 0.05). However, no significant correlation was found between caspase-1 and disease duration. Inflammasomes may participate in the inflammatory process of OME. IL-1β and IL-18 levels in the MEE decreased over time.
本研究旨在评估分泌性中耳炎（OME）患儿中耳积液中半胱天冬酶-1的表达以及细胞因子（如白细胞介素（IL）-1β和IL-18）的半胱天冬酶-1依赖性加工过程，以确定炎性小体在OME中的潜在作用。本研究纳入了29例因OME计划行鼓膜切开置管术的患儿。手术过程中收集中耳积液（MEE）。使用酶联免疫吸附测定试剂盒检测半胱天冬酶-1、IL-1β和IL-18。比较了黏液性和浆液性MEE患儿以及有和无鼓膜置管史患儿的上述指标水平。所有样本均检测到半胱天冬酶-1、IL-1β和IL-18。黏液性样本和浆液性样本之间的半胱天冬酶-1、IL-1β和IL-18水平无显著差异。有和无鼓膜置管史组之间的半胱天冬酶-1、IL-1β和IL-18水平未发现统计学意义。IL-1β和IL-18与OME病程呈显著负相关（<0.05）。然而，未发现半胱天冬酶-1与病程之间存在显著相关性。炎性小体可能参与了OME的炎症过程。MEE中IL-1β和IL-18水平随时间下降。
