Department of Otorhinolaryngology-Head and Neck Surgery, The Catholic University of Korea School of Medicine, Seoul, Korea.
Clin Exp Otorhinolaryngol. 2010 Mar;3(1):18-23. doi: 10.3342/ceo.2010.3.1.18. Epub 2010 Mar 30.
While other cytokines are known to be associated with otitis media with effusion (OME), the involvement of heat shock protein 70 (HSP70) in middle ear effusion (MEE) is unknown. This study was undertaken to investigate the possibility of there being a HSP70 expression in human MEE and to determine its potential role as a cytokine in OME.
The levels of HSP70, tumor necrosis factor-alpha and interleukin-1beta were measured by enzyme-linked immunosorbent assay in the effusion of different groups of OME patient following collection of the MEE using our new collection system. The clinical characteristics of the OME patients and the MEE status were analyzed.
HSP70 was expressed in all the types of MEE. The mucous and seromucous effusions showed higher HSP70 levels than that of the serous effusion. The HSP70 level was correlated with the levels of tumor necrosis factor (TNF)-alpha and interleukin (IL)-1beta in the effusions. The positive correlations between HSP70, TNF-alpha and IL-1beta were statistically significant (P<0.05).
The highly elevated level of HSP70 in the seromucous and mucous effusions implicates this protein in the chronicity of OME.
虽然已知其他细胞因子与分泌性中耳炎（OME）有关，但热休克蛋白 70（HSP70）在中耳积液（MEE）中的参与情况尚不清楚。本研究旨在探讨 HSP70 在人 MEE 中表达的可能性，并确定其作为 OME 中细胞因子的潜在作用。
使用我们的新收集系统收集 MEE 后，通过酶联免疫吸附试验测量不同 OME 患者组的 HSP70、肿瘤坏死因子-α和白细胞介素-1β的水平。分析 OME 患者的临床特征和 MEE 状态。
HSP70 在所有类型的 MEE 中均有表达。黏液性和浆黏液性积液中的 HSP70 水平高于浆液性积液。HSP70 水平与积液中的肿瘤坏死因子（TNF）-α和白细胞介素（IL）-1β水平相关。HSP70、TNF-α 和 IL-1β 之间存在显著的正相关（P<0.05）。
浆黏液性和黏液性积液中 HSP70 水平的高度升高表明该蛋白与 OME 的慢性化有关。
