Firtel M, Fillery E D
Faculty of Dentistry, University of Toronto, Ontario, Canada.
J Dent Res. 1988 Jan;67(1):15-20. doi: 10.1177/00220345880670010101.
A total of 18 monoclonal antibodies was raised against whole cells of Actinomyces viscosus and Actinomyces naeslundii. The monoclonal antibodies were used to determine the cross-reacting patterns among 26 strains of these species. Eleven different antigenic determinants were found. The specificity profiles of the antibodies indicated that the antigenic determinants of A. viscosus and A. naeslundii were arranged in a complicated mosaic. Extensive cross-reactions occurred between A. viscosus strains and strains of "typical" and "atypical" A. naeslundii. However, cross-reactions were rare between the two groups of A. naeslundii. A. viscosus appears to occupy a "middle position" between the two A. naeslundii groups. In addition to their value in seroclassification, some of the monoclonal antibodies were found to be useful in the identification of these species. One monoclonal antibody appeared to be selective for the "typical" A. naeslundii group. A. viscosus and "atypical" A. naeslundii-specific antibodies were also found, though they did not label every strain in their respective clusters. A. viscosus detection might be improved if mixtures of monoclonal antibodies were used.
共制备了18种针对黏性放线菌和内氏放线菌全细胞的单克隆抗体。这些单克隆抗体用于确定这两个菌种的26个菌株之间的交叉反应模式。发现了11种不同的抗原决定簇。抗体的特异性谱表明，黏性放线菌和内氏放线菌的抗原决定簇呈复杂的镶嵌排列。黏性放线菌菌株与“典型”和“非典型”内氏放线菌菌株之间发生了广泛的交叉反应。然而，两组内氏放线菌之间的交叉反应很少见。黏性放线菌似乎处于两组内氏放线菌之间的“中间位置”。除了在血清分类中的价值外，还发现一些单克隆抗体可用于这些菌种的鉴定。一种单克隆抗体似乎对“典型”内氏放线菌组具有选择性。还发现了黏性放线菌和“非典型”内氏放线菌特异性抗体，尽管它们并未标记各自菌群中的每一个菌株。如果使用单克隆抗体混合物，黏性放线菌的检测可能会得到改善。
