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大肠杆菌K-12中对大肠杆菌素抗性的遗传学:B组大肠杆菌素之间的交叉抗性

Genetics of resistance to colicins in Escherichia coli K-12: cross-resistance among colicins of group B.

作者信息

Davies J K, Reeves P

出版信息

J Bacteriol. 1975 Jul;123(1):96-101. doi: 10.1128/jb.123.1.96-101.1975.

Abstract

This paper describes the isolation of mutants resistant to colicins of group B (i.e., colicins B, D, G, H, Ia, Ib, M, Q, S1, and V). The 145 mutants studied in detail can be divided into nine phentopyic classes, based on their colicin-resistance patterns. They include the previously isolated tonA, tonB exbB and cir mutants. Each of the different phenotypic classes of mutants has been partially characterized, and some approximately mapped. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis has revealed substantial changes in the composition of the outer membrane proteins of exbB and tonB mutants.

摘要

本文描述了对B组大肠杆菌素(即大肠杆菌素B、D、G、H、Ia、Ib、M、Q、S1和V)具有抗性的突变体的分离。根据其对大肠杆菌素的抗性模式,对详细研究的145个突变体可分为九个表型类别。它们包括先前分离出的tonA、tonB exbB和cir突变体。已对不同表型类别的每个突变体进行了部分表征,并对其中一些进行了大致定位。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳显示,exbB和tonB突变体的外膜蛋白组成发生了显著变化。

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