Behr B, Fisch J D, Racowsky C, Miller K, Pool T B, Milki A A
Department of Gynecology and Obstetrics, Stanford University School of Medicine, California 94305, USA.
J Assist Reprod Genet. 2000 Jul;17(6):349-51. doi: 10.1023/a:1009461213139.
To examine the rate of monozygotic twinning associated with blastocyst transfer using commercially available, cell-free culture systems with unmanipulated blastocysts.
A retrospective analysis was conducted in multiple private and academic infertility centers throughout the United States, of 199 pregnant patients following in vitro fertilization (IVF) blastocyst embryo transfer (ET). Human embryos obtained through standard IVF stimulation protocols were cultured in commercially available, cell-free media systems and transferred as blastocysts. The main outcome measure was the rate of monozygotic twinning.
A total of 199 blastocyst-ET pregnancies were achieved during the study period at the fertility centers examined. Monozygotic twinning was noted in 10/199 (5%) of these pregnancies. All were monochorionic diamnionic.
Monozygotic twinning previously has been reported following IVF, especially in relation to assisted hatching. While blastocyst transfer has been available for many years using coculture, there have been no published multicenter reports of monozygotic twinning associated with unmanipulated blastocysts. In a multicenter analysis, a definite increase in monozygotic twinning was seen following blastocyst-ET. We believe this phenomenon is real and that this information should be considered when counseling patients for treatment.
使用市售的无细胞培养系统培养未经处理的囊胚，研究与囊胚移植相关的单卵双胎发生率。
在美国多个私立和学术性不孕不育中心进行了一项回顾性分析，研究对象为199例接受体外受精（IVF）囊胚胚胎移植（ET）后的孕妇。通过标准IVF刺激方案获得的人类胚胎在市售的无细胞培养基系统中培养，并作为囊胚进行移植。主要观察指标为单卵双胎发生率。
在研究期间，在所检查的生育中心共实现了199例囊胚ET妊娠。在这些妊娠中，有10/199（5%）出现单卵双胎。所有均为单绒毛膜双羊膜囊。
此前已有IVF后单卵双胎的报道，尤其是与辅助孵化有关。虽然使用共培养进行囊胚移植已有多年，但尚无关于未经处理的囊胚相关单卵双胎的多中心报道。在一项多中心分析中，囊胚ET后单卵双胎明显增加。我们认为这一现象是真实存在的，在为患者提供治疗咨询时应考虑这一信息。
