Seki N, Yoshikawa T, Hattori A, Miyajima N, Muramatsu M, Saito T
Genome Research Group, National Institute of Radiological Sciences, Anagawa, Chiba, Japan.
J Hum Genet. 2000;45(5):309-14. doi: 10.1007/s100380070023.
Members of the RAB protein family are important regulators of vesicular fusion and trafficking. A putative new member of the RAB family of genes was identified through a public database search, and its full-length cDNA was isolated from a human fetal brain cDNA library. The predicted protein product of the gene consists of 190 amino acid residues and has 87% identity with rat Rab26. Thus, we designated this gene as the human RAB26-related gene. Reverse transcription-coupled polymerase chain reaction (RT-PCR) demonstrated that the RAB26-related messenger RNA was predominantly expressed in adult and fetal brain. Furthermore, an RT-PCR experiment for brain subregions showed that the mRNA was highly expressed in the amygdala, cerebellum, caudate nucleus, and hippocampus. By PCR-based analysis with both a human/rodent monochromosomal hybrid cell panel and a radiation hybrid panel, the gene was mapped to the chromosome 16p13.3 region between markers WI-7742 and WI-3061. The RAB26-related gene consists of eight exons that span about 44kb of the genome DNA.
RAB蛋白家族成员是囊泡融合和运输的重要调节因子。通过公共数据库搜索鉴定出RAB基因家族的一个假定新成员，并从人胎脑cDNA文库中分离出其全长cDNA。该基因预测的蛋白质产物由190个氨基酸残基组成，与大鼠Rab26具有87%的同一性。因此，我们将该基因命名为人类RAB26相关基因。逆转录偶联聚合酶链反应（RT-PCR）表明，RAB26相关信使RNA主要在成体和胎儿脑中表达。此外，针对脑亚区域的RT-PCR实验表明，该mRNA在杏仁核、小脑、尾状核和海马体中高度表达。通过使用人/啮齿动物单染色体杂交细胞系和辐射杂种细胞系进行基于PCR的分析，该基因被定位到16号染色体p13.3区域，位于标记WI-7742和WI-3061之间。RAB26相关基因由八个外显子组成，跨越约44kb的基因组DNA。
