Fukutani Y, Cairns N J, Shiozawa M, Sasaki K, Sudo S, Isaki K, Lantos P L
Department of Neuropsychiatry, Fukui Medical University, Japan.
Psychiatry Clin Neurosci. 2000 Oct;54(5):523-9. doi: 10.1046/j.1440-1819.2000.00747.x.
To explore more fully the relationship between neuronal death and neurofibrillary degeneration, unaffected neurons, intracellular neurofibrillary tangles (i-NFT) and extracellular NFT (e-NFT) in 22 patients with late-onset sporadic Alzheimer's disease (AD) were morphometrically evaluated in eight subdivisions of the hippocampal cortex, using the Gallyas hematoxylin-eosin stain. The subdivisions examined included CA4, CA3, CA2, CA1 (CA: cornu ammonis), prosubiculum (PRO), subiculum and presubiculum (PRE), parasubiculum (PARA) and the entorhinal cortex (ENT). The unaffected neuron density was significantly lower and both i-NFT and e-NFT densities were significantly higher in subdivisions other than CA4 and CA3 in AD patients compared with those in the aged controls. Unaffected neuron density was significantly, inversely correlated with e-NFT density and with total NFT density in all subdivisions except for PRE in AD patients. Especially in CA2, CA1, PRO and ENT, there were strong correlations between the neuron density and these NFT densities. Both unaffected neuron and e-NFT densities in CA1 and ENT were significantly correlated with the disease duration. The i/e-NFT ratio, an index of the degree and/or rate of progress of neuronal death via neurofibrillary degeneration, showed the lowest value in ENT in AD patients. The findings suggest that neuronal death via neurofibrillary degeneration starts earliest and/or most rapidly progresses in ENT. Furthermore, the i/e-NFT ratios in both ENT and CA1 were significantly correlated with the disease duration, suggesting that the neuronal death pattern in the two subdivisions parallels disease progression.
为更全面地探究神经元死亡与神经原纤维变性之间的关系，我们使用Gallyas苏木精-伊红染色法，对22例晚发性散发性阿尔茨海默病（AD）患者海马皮质的八个亚区中未受影响的神经元、细胞内神经原纤维缠结（i-NFT）和细胞外NFT（e-NFT）进行了形态计量学评估。所检查的亚区包括CA4、CA3、CA2、CA1（CA：海马角）、前下托（PRO）、下托、前下托（PRE）、旁下托（PARA）和内嗅皮质（ENT）。与老年对照组相比，AD患者中除CA4和CA3外的其他亚区未受影响的神经元密度显著降低，而i-NFT和e-NFT密度均显著升高。在AD患者中，除PRE外的所有亚区，未受影响的神经元密度与e-NFT密度以及总NFT密度均呈显著负相关。特别是在CA2、CA1、PRO和ENT中，神经元密度与这些NFT密度之间存在很强的相关性。CA1和ENT中未受影响的神经元密度和e-NFT密度均与病程显著相关。i/e-NFT比值是通过神经原纤维变性导致神经元死亡的程度和/或进展速度的指标，在AD患者的ENT中显示出最低值。这些发现表明，通过神经原纤维变性导致的神经元死亡在ENT中最早开始和/或进展最为迅速。此外，ENT和CA1中的i/e-NFT比值均与病程显著相关，表明这两个亚区的神经元死亡模式与疾病进展平行。
