Yu Q, Lu Y, Nerurkar V R, Yanagihara R
Retrovirology Research Laboratory, Pacific Biomedical Research Center, University of Hawaii at Manoa, Honolulu 96816, USA.
Arch Virol. 2000;145(12):2669-76. doi: 10.1007/s007050070015.
A 1,632-bp fragment, flanking the original 483-bp region of the DNA polymerase gene of a novel herpesvirus found in tissues of green turtles (Chelonia mydas) with fibropapilloma, was amplified from the circularized EcoRI-cut DNA extracted from tumor tissues by inverse PCR. The resultant 2,019-bp partial sequence of the DNA polymerase gene of the newfound herpesvirus, including the original 483-bp region, showed a high degree of homology at both the nucleotide and amino acid levels with that of other human and animal herpesviruses. Phylogenetic analysis confirmed that this novel herpesvirus belonged to the Alphaherpesvirinae subfamily.
从患有纤维乳头瘤的绿海龟（蠵龟）组织中发现的一种新型疱疹病毒的DNA聚合酶基因原始483 bp区域侧翼的一个1,632 bp片段，通过反向PCR从肿瘤组织提取的经EcoRI酶切环化的DNA中扩增得到。新发现的疱疹病毒DNA聚合酶基因的所得2,019 bp部分序列，包括原始的483 bp区域，在核苷酸和氨基酸水平上与其他人类和动物疱疹病毒的序列具有高度同源性。系统发育分析证实，这种新型疱疹病毒属于α疱疹病毒亚科。
