Pinto de Andrade M, Schönian G, Forche A, Rosado L, Costa I, Müller M, Presber W, Mitchell T G, Tietz H J
Institut für Mikrobiologie und Hygiene, Universitätsklinikum Charité, Humboldt-Universität Berlin, Germany.
Int J Med Microbiol. 2000 Mar;290(1):97-104. doi: 10.1016/s1438-4221(00)80112-5.
PCR fingerprinting with single non-specific primers was used to type vaginal isolates of C. albicans from Portugal, Angola, Madagascar, and two regions of Germany (Berlin and Munich). In addition to analysing isolates that exhibited the normal biotype of C. albicans, the study included atypical strains that failed to assimilate glucosamine and N-acetylglucosamine, which were isolated from women in Angola and Madagascar. A total of 212 strains of C. albicans were studied, representing 87 different multi-locus genotypes. The genotypes of strains from each geographical population were highly similar but not identical. There was one exception: a strain from Portugal grouped with the typical strains from Angola. The typical and especially the atypical populations from Africa displayed less genotype variation than the populations from Europe. The Portuguese samples exhibited the greatest genotypic heterogeneity. Distance analysis (UPGMA) revealed a statistically weak correlation between genotype and geographical origin of the C. albicans isolates.
使用单条非特异性引物进行PCR指纹分析，对来自葡萄牙、安哥拉、马达加斯加以及德国两个地区（柏林和慕尼黑）的白色念珠菌阴道分离株进行分型。除了分析表现出白色念珠菌正常生物型的分离株外，该研究还包括从安哥拉和马达加斯加女性中分离出的无法同化氨基葡萄糖和N - 乙酰氨基葡萄糖的非典型菌株。总共研究了212株白色念珠菌，代表87种不同的多位点基因型。每个地理群体的菌株基因型高度相似但不完全相同。有一个例外：一株来自葡萄牙的菌株与来自安哥拉的典型菌株归为一组。非洲的典型群体尤其是非典型群体表现出的基因型变异比欧洲群体少。葡萄牙的样本表现出最大的基因型异质性。距离分析（UPGMA）显示白色念珠菌分离株的基因型与地理来源之间存在统计学上较弱的相关性。
