Department of Obstetrics and Gynecology, Faculty of Medicine, University of Çukurova, Adana, Turkey.
Mycopathologia. 2013 Apr;175(3-4):287-94. doi: 10.1007/s11046-013-9643-2. Epub 2013 Apr 2.
Recent studies have clearly defined the vaginopathic Candida albicans strains that cause severe vulvovaginal candidiasis (VVC). Therefore, genotyping C. albicans isolates may predict the success of and assist in choosing the appropriate antifungal therapy. The purpose of this study was to compare the genotypes of C. albicans isolates causing VVC with those found in asymptomatic healthy pregnant and non-pregnant women in Adana, Turkey, as well as the antifungal susceptibility profiles of these isolates. A total of 216 independent C. albicans isolates were genotyped by allelic combination based on the microsatellite marker analysis of one such microsatellite, present in the promoter region of the elongation factor 3-encoding gene (CEF3) of C. albicans. The susceptibility testing profiles of all of the isolates against five antifungals and boric acid were obtained retrospectively from our laboratory records. We identified 20 genotypes on the basis of different allelic combinations at the CEF3 locus with a discriminatory power of 0.85. Genotypes 136-144 and 126-135 were present in 50 % of the isolates. No differences existed in the genotypic profiles of fungal isolates between pregnant and non-pregnant women. Remarkably, we did not find a single vaginopathic genotype. All of the isolates were susceptible to amphotericin B and 5-fluorocytosine, and the fluconazole and ketoconazole resistance rates were 0.9 and 3.7 %, respectively. Therefore, we did not find any correlation between genotype, severity of VVC, and antifungal resistance (P > 0.05). Even so, additional molecular data may provide new insights into the management of VVC.
最近的研究清楚地定义了引起严重外阴阴道念珠菌病（VVC）的阴道病原体白念珠菌菌株。因此，对白念珠菌分离株进行基因分型可能有助于预测治疗的成功并选择合适的抗真菌治疗。本研究的目的是比较导致 VVC 的白念珠菌分离株的基因型与在土耳其阿达纳的无症状健康孕妇和非孕妇中的基因型，并比较这些分离株的抗真菌药敏谱。共对 216 株白念珠菌独立分离株进行基因分型，基于微卫星标记分析一个微卫星，该微卫星位于白念珠菌伸长因子 3 编码基因（CEF3）的启动子区域。所有分离株对五种抗真菌药物和硼酸的敏感性测试谱均从我们的实验室记录中回顾性获得。我们根据 CEF3 基因座的不同等位基因组合鉴定了 20 种基因型，其区分能力为 0.85。基因型 136-144 和 126-135 存在于 50%的分离株中。孕妇和非孕妇之间真菌分离株的基因型谱没有差异。值得注意的是，我们没有发现单一的阴道病原体基因型。所有分离株均对两性霉素 B 和 5-氟胞嘧啶敏感，氟康唑和酮康唑的耐药率分别为 0.9%和 3.7%。因此，我们没有发现基因型、VVC 严重程度和抗真菌耐药性之间存在任何相关性（P > 0.05）。即便如此，额外的分子数据可能为 VVC 的管理提供新的见解。
