Vassallo J, Derchain S F, Pinto G A, Martinez E Z, Syrjänen K J, Andrade L A
Department of Anatomic Pathology, State University of Campinas (UNICAMP), São Paulo, Brazil.
Int J Gynaecol Obstet. 2000 Oct;71(1):45-8. doi: 10.1016/s0020-7292(00)00248-4.
Cervical intraepithelial lesions due to HPV infection are common in Brazil. An understanding of the mechanisms of the interaction between HPV and host factors is still incomplete. In spite of the high incidence of cervical cancer in Brazil, such studies with Brazilian patients are scarce. The purpose of this study was to correlate the presence of high-risk types of HPV and expression of p53 protein, grade of cervical lesion, age, high-risk sexual behaviors and smoking. It was also intended to establish whether p53 expression might be useful as a marker for CIN progression.
HPV detection was performed on paraffin sections using biotin-labeled probes by in situ hybridization. p53 protein expression was evaluated by immunohistochemistry.
Seventy-eight patients with cervical dysplasia were included in the study. CIN 1 was diagnosed in 38 cases, and CIN 2+3 in 40 cases. High-risk HPV was detected in 42 patients. No correlation was found between the grade of cervical lesion or the presence of HPV and smoking, and high-risk sexual behavior. Expression of p53 was significantly higher in CIN 1, as compared with CIN 2+3, but did not correlate with HPV status.
Higher expression of p53 protein in early lesions supports the hypothesis of a partially protective role of the wild-type p53 in early stages of cervical lesions.
在巴西，由人乳头瘤病毒（HPV）感染引起的宫颈上皮内病变很常见。对HPV与宿主因素之间相互作用机制的了解仍不完整。尽管巴西宫颈癌发病率很高，但针对巴西患者的此类研究却很少。本研究的目的是将高危型HPV的存在与p53蛋白表达、宫颈病变分级、年龄、高危性行为和吸烟进行关联。同时还旨在确定p53表达是否可作为宫颈上皮内瘤变（CIN）进展的标志物。
通过原位杂交，使用生物素标记的探针在石蜡切片上进行HPV检测。通过免疫组织化学评估p53蛋白表达。
78例宫颈发育异常患者纳入本研究。38例诊断为CIN 1，40例诊断为CIN 2+3。42例患者检测到高危型HPV。未发现宫颈病变分级、HPV的存在与吸烟及高危性行为之间存在关联。与CIN 2+3相比，CIN 1中p53的表达显著更高，但与HPV状态无关。
早期病变中p53蛋白的高表达支持野生型p53在宫颈病变早期具有部分保护作用这一假说。
