春雷霉素与大肠杆菌核糖体的结合。

The binding of kasugamycin to the Escherichia coli ribosomes.

作者信息

Okuyama A, Tanaka N, Komai T

出版信息

J Antibiot (Tokyo). 1975 Nov;28(11):903-5. doi: 10.7164/antibiotics.28.903.

DOI:10.7164/antibiotics.28.903

Abstract

The binding of kasugamycin to the E. coli ribosomes has been demonstrated by equilibrium dialysis. Kasugamycin binds to the 70S ribosomes in a molar ratio of 1:1. The association constant is approximately 6 X 10(-4) M-1. The antibiotic binds to the 30S subunit as well as to 70S ribosomes, but only slightly to the 50S subunit. The binding of kasugamycin is not significantly affected by the presence of other aminolgycoside antibiotics: streptomycin, kanamycin. or gentamicin. Kasugamycin does not bind to ribosomes derived from a kasugamycin-resistant mutant, ksgC.

摘要

通过平衡透析已证明春日霉素与大肠杆菌核糖体的结合。春日霉素以1:1的摩尔比与70S核糖体结合。缔合常数约为6×10⁻⁴ M⁻¹。该抗生素与30S亚基以及70S核糖体结合，但与50S亚基的结合很微弱。其他氨基糖苷类抗生素（链霉素、卡那霉素或庆大霉素）的存在对春日霉素的结合没有显著影响。春日霉素不与源自春日霉素抗性突变体ksgC的核糖体结合。