酿酒酵母的脂肪酸去饱和酶突变体
Fatty acid desaturase mutants of Saccharomyces cerevisiae.
作者信息
Keith A D, Resnick M R, Haley A B
出版信息
J Bacteriol. 1969 May;98(2):415-20. doi: 10.1128/jb.98.2.415-420.1969.
Abstract
Genetic and biochemical analyses were conducted on fatty acid mutants of yeast deficient for Delta(9)-desaturase activity in the production of palmitoleate and oleate. Two genetic loci were observed and two others are inferred; three of these were represented by respiratory-deficient (petite) strains. All strains were incapable of converting palmitate to palmitoleate and stearate to oleate whether the direct precursor or acetate was followed. All strains were capable of acylating both de novoproduced fatty acids and oleate taken up from the medium into phospholipids and neutral lipids. Two revertants were analyzed which differed in their ability to produce palmitoleate and oleate.
摘要
对酵母脂肪酸突变体进行了遗传和生化分析，这些突变体在棕榈油酸和油酸的生产中缺乏Δ(9)-去饱和酶活性。观察到两个基因位点，另外推断出两个；其中三个由呼吸缺陷型（小菌落）菌株代表。无论追踪的是直接前体还是乙酸盐，所有菌株都无法将棕榈酸转化为棕榈油酸，将硬脂酸转化为油酸。所有菌株都能够将新产生的脂肪酸和从培养基中摄取的油酸酰化到磷脂和中性脂质中。分析了两个回复突变体，它们在产生棕榈油酸和油酸的能力上有所不同。
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