Eurofins¦Wiertz-Eggert-Jörissen, Neuländer Kamp 1, 21079, Hamburg, Germany,
Mycotoxin Res. 2008 Jun;24(2):98-104. doi: 10.1007/BF02985287.
A study was conducted to determine the distribution of deoxynivalenol (DON) and ochratoxin A (OTA) in a lot of 261 of wheat kernels. Within this study, two different sampling and sample preparation strategies were carried out. On the one hand, following the official commission regulation 401/2006/EC, an aggregate sample out of 100 incremental samples was build, homogenized and prepared for laboratory analysis. On the other hand each individual subsample was investigated for its deoxynivalenol and ochratoxin A content. The determined concentration of DON in the individual samples was in a range from 830 up to 2655 (μg/kg, for OTA results ranged from < 0.2 up to 8.6 μg/kg. Thus, a coefficient of variance of 25% for DON and 200% for OTA was achieved. From this, a spot formation for OTA was observed and the average value of the 100 incremental samples did not correspond to the achieved value for the aggregate sample. While the DON contamination at this concentration range seems to be more even, consequently the result of the aggregate sample was in accordance with the average value.In addition a sample communition study was performed to answer the question whether the time consuming process of grinding of the whole aggregate sample is necessary or not. The results of this study show that contamination of whole wheat kernels with DON is at the same level within a 1 kg sample (CV 16%), while OTA contamination shows high variability (CV 94%). At least for OTA this study indicated that an extensive and complete sample communition of the high volume aggregate sample is necessary.
一项研究旨在确定 261 份小麦籽粒中脱氧雪腐镰刀菌烯醇（DON）和赭曲霉毒素 A（OTA）的分布。在这项研究中，采用了两种不同的采样和样品制备策略。一方面，根据官方委员会法规 401/2006/EC，从 100 个增量样品中构建了一个总样品，进行了混合并准备用于实验室分析。另一方面，对每个单独的次级样品进行了脱氧雪腐镰刀菌烯醇和赭曲霉毒素 A 含量的检测。个别样品中 DON 的测定浓度范围为 830 至 2655μg/kg（对于 OTA 的结果范围为<0.2 至 8.6μg/kg）。因此，DON 的变异系数为 25%，OTA 的变异系数为 200%。由此观察到 OTA 的点状形成，并且 100 个增量样品的平均值与总样品的平均值不对应。虽然在这个浓度范围内 DON 的污染似乎更加均匀，但总样品的结果与平均值一致。此外，还进行了样品粉碎研究，以回答是否需要对整个总样品进行耗时的粉碎过程。该研究的结果表明，整个小麦籽粒中 DON 的污染水平在 1 公斤样品中是相同的（CV 为 16%），而 OTA 的污染则具有很高的变异性（CV 为 94%）。至少对于 OTA 来说，这项研究表明，对大容量总样品进行广泛而完整的样品粉碎是必要的。
