Kanazawa Y, Kuramata T
Jpn J Antibiot. 1975 Dec;28(6):745-52.
The MIC''s of sulfobenzylpenicillin (SB-PC) were determined by the two-fold serial agar-dilution method for 108 bacterial strains of 21 species. The diameters of inhibition zones of these bacterial strains by the 30 mug and 200 mug SB-PC discs were also measured. The relation between the MIC and the diameter of the inhibition zone was found to be expressed as a primary regression line in all cases of the conventional method (cultured for about 16 hours), delayed assay method (cultured for about 24 hours) and rapid methods (5 to approximately 6 hours and 3 to approximately 4 hours culture m ethods). Thus, it was confirmed that the single-disc method can be employed for the susceptibility test of SB-PC. Subsequently, variations of MICs obtained by the disc-diffusion method were compared with those obtained by the serial agar-dilution method.
采用二倍稀释琼脂平板法测定了21种108株细菌对磺苄西林（SB-PC）的最低抑菌浓度（MIC）。还测定了这些菌株对含30μg和200μg SB-PC纸片的抑菌圈直径。在常规方法（培养约16小时）、延迟检测法（培养约24小时）和快速方法（培养5至约6小时及3至约4小时）的所有情况下，均发现MIC与抑菌圈直径之间的关系可用一元回归线表示。因此，证实单纸片法可用于SB-PC的药敏试验。随后，将纸片扩散法获得的MIC变化与系列琼脂稀释法获得的MIC变化进行了比较。
