Kanazawa Y, Kuramata T
Jpn J Antibiot. 1976 Aug;29(8):725-30.
The MICs of ribostamycin (RSM) were determined by the two-fold serial agar-dilution method of 109 bacterial strains of 20 species. The diameters of inhibition zones of these bacterial strains by the 50 mug RSM disc were also measured. The relation between the MIC and the diameter of the inhibition zone was found to be expressed as a primary regression line in all cases of the conventional method (cultured for about 16 hours), delayed assay method (cultured for about 24 hours) and rapid methods (5 approximately 6 hours and 3 approximately 4 hours culture methods). Thus, it was confirmed that the single-disc method can be employed for the susceptibility test of RSM. Subsequently, variations of MICs obtained by the disc-diffusion method were compared with those obtained by the serial agar-dilution method.
采用二倍稀释琼脂平板法测定了20种109株细菌对核糖霉素（RSM）的最低抑菌浓度（MIC）。同时测定了50μg RSM纸片对这些菌株的抑菌圈直径。结果发现，在常规方法（培养约16小时）、延迟检测方法（培养约24小时）和快速方法（5～6小时及3～4小时培养方法）的所有情况下，MIC与抑菌圈直径的关系均可用一条一元回归直线表示。因此，证实单纸片法可用于RSM的药敏试验。随后，将纸片扩散法获得的MIC变化与系列琼脂稀释法获得的MIC变化进行了比较。
