Knox K K, Brewer J H, Henry J M, Harrington D J, Carrigan D R
Institute for Viral Pathogenesis, Milwaukee, WI 53226, USA.
Clin Infect Dis. 2000 Oct;31(4):894-903. doi: 10.1086/318141. Epub 2000 Oct 20.
By means of immunohistochemical staining, cells actively infected with human herpesvirus 6 (HHV-6) were found in central nervous system tissues from 8 (73%) of 11 patients with definite multiple sclerosis (MS). Interestingly, 17 (90%) of 19 tissue sections showing active demyelination were positive for HHV-6-infected cells compared with only 3 (13%) of 23 tissue sections free of active disease (P<.0001). Central nervous system tissues from 2 of 28 normal persons and patients with other inflammatory demyelinative diseases were positive for HHV-6-infected cells (P<.0001), and the 2 positive cases were diagnosed as having HHV-6 leukoencephalitis. By use of a rapid culture assay, blood samples from 22 (54%) of 41 patients with definite MS were found to contain active HHV-6 infections, compared with 0 of 61 normal controls (P<.0001). No significant difference was found between HHV-6 viremia-positive and HHV-6 viremia-negative MS patients with respect to type of disease (relapsing/remitting or progressive). In contrast, patients with active HHV-6 viremia were significantly younger and had shorter durations of disease than did HHV-6 viremia-negative patients.
通过免疫组织化学染色发现，在11例确诊为多发性硬化症（MS）的患者中，有8例（73%）的中枢神经系统组织中存在活跃感染人疱疹病毒6型（HHV-6）的细胞。有趣的是，在19个显示有活跃脱髓鞘的组织切片中，有17个（90%）对HHV-6感染细胞呈阳性，而在23个无活跃病变的组织切片中只有3个（13%）呈阳性（P<0.0001）。在28名正常人和其他炎症性脱髓鞘疾病患者中，有2人的中枢神经系统组织对HHV-6感染细胞呈阳性（P<0.0001），这2例阳性病例被诊断为患有HHV-6白质脑炎。通过快速培养检测发现，41例确诊为MS的患者中有22例（54%）的血液样本含有活跃的HHV-6感染，而61名正常对照者中无一例阳性（P<0.0001）。在疾病类型（复发/缓解型或进展型）方面，HHV-6病毒血症阳性和阴性的MS患者之间未发现显著差异。相比之下，HHV-6病毒血症活跃的患者比HHV-6病毒血症阴性的患者明显年轻，病程也更短。
