Durbin E A, Gregory M S, Messingham K A, Fontanilla C V, Duffner L A, Kovacs E J
Department of Cell Biology, Neurobiology, and Anatomy, Loyola University Medical Center, 2160 South First Avenue, Maywood, IL 60153, USA.
Cytokine. 2000 Nov;12(11):1669-75. doi: 10.1006/cyto.2000.0768.
Following traumatic injury, patients suffer from compromised immunity increasing their susceptibility to infection. Previous studies from this laboratory demonstrated that female BALB/c mice subjected to a 15% total body surface area (TBSA) scald injury exhibit a decrease in cell-mediated immunity 10 days post-burn. Studies described herein revealed that concanavalin A (Con A; 2 microg/ml)-stimulated splenocytes from sham treated animals produced 3557+/-853 pg/ml of IFN-gamma while splenocytes from burn injured animals released two-fold more cytokine (P<0.05). To determine whether leukocyte production of IFN-gamma was under the influence of macrophages, splenic macrophage supernatants generated from burned animals were incubated with splenic lymphocytes from sham and burn animals. The amount of IFN-gamma released by lymphocytes from sham animals increased when cultured with macrophages from burned mice (P<0.05). This suggests that the increase in IFN-gamma production by unfractionated splenocytes in burned mice relative to sham treated animals is macrophage-dependent. Macrophage supernatants from burned mice released twice as much IL-6 as supernatants from sham animals (P<0.05), and when IL-6 was blocked in vivo, the amount of IFN-gamma production in burned mice decreased to sham levels (P<0.05). Thus, IL-6 mediates IFN-gamma production following burn.
创伤性损伤后，患者免疫力受损，增加了感染易感性。本实验室先前的研究表明，遭受15%体表面积（TBSA）烫伤的雌性BALB/c小鼠在烧伤后10天细胞介导的免疫功能下降。本文所述研究表明，来自假手术处理动物的伴刀豆球蛋白A（Con A；2微克/毫升）刺激的脾细胞产生3557±853皮克/毫升的干扰素-γ，而来自烧伤动物的脾细胞释放的细胞因子则多出两倍（P<0.05）。为了确定干扰素-γ的白细胞产生是否受巨噬细胞影响，将烧伤动物产生的脾巨噬细胞上清液与假手术和烧伤动物的脾淋巴细胞一起孵育。与烧伤小鼠的巨噬细胞共培养时，假手术动物淋巴细胞释放的干扰素-γ量增加（P<0.05）。这表明，与假手术处理动物相比，烧伤小鼠中未分离的脾细胞产生的干扰素-γ增加是巨噬细胞依赖性的。烧伤小鼠的巨噬细胞上清液释放的白细胞介素-6是假手术动物上清液的两倍（P<0.05），并且当体内阻断白细胞介素-6时，烧伤小鼠中干扰素-γ的产生量降至假手术水平（P<0.05）。因此，白细胞介素-6介导烧伤后的干扰素-γ产生。
