Baumgardner J E, Choi I C, Vonk-Noordegraaf A, Frasch H F, Neufeld G R, Marshall B E
Department of Anesthesia, University of Pennsylvania, Philadelphia, Pennsylvania 19104-4283, USA.
J Appl Physiol (1985). 2000 Nov;89(5):1699-708. doi: 10.1152/jappl.2000.89.5.1699.
We developed micropore membrane inlet mass spectrometer (MMIMS) probes to rapidly measure inert-gas partial pressures in small blood samples. The mass spectrometer output was linearly related to inert-gas partial pressure (r(2) of 0.996-1.000) and was nearly independent of large variations in inert-gas solubility in liquid samples. We infused six inert gases into five pentobarbital-anesthetized New Zealand rabbits and used the MMIMS system to measure inert-gas partial pressures in systemic and pulmonary arterial blood and in mixed expired gas samples. The retention and excretion data were transformed into distributions of ventilation-to-perfusion ratios (V(A)/Q) with the use of linear regression techniques. Distributions of V(A)/Q were unimodal and broad, consistent with prior reports in the normal rabbit. Total blood sample volume for each VA/Q distribution was 4 ml, and analysis time was 8 min. MMIMS provides a convenient method to perform the multiple inert-gas elimination technique rapidly and with small blood sample volumes.
我们开发了微孔膜进样质谱仪（MMIMS）探头，用于快速测量小份血液样本中的惰性气体分压。质谱仪输出与惰性气体分压呈线性相关（r²为0.996 - 1.000），并且几乎不受液体样本中惰性气体溶解度大幅变化的影响。我们将六种惰性气体注入五只戊巴比妥麻醉的新西兰兔体内，并使用MMIMS系统测量体循环和肺动脉血液以及混合呼出气体样本中的惰性气体分压。利用线性回归技术将潴留和排泄数据转换为通气/灌注比（V(A)/Q）分布。V(A)/Q分布呈单峰且宽泛，与正常兔的先前报道一致。每个V(A)/Q分布的总血样体积为4毫升，分析时间为8分钟。MMIMS提供了一种便捷的方法，能够快速且使用少量血样进行多种惰性气体消除技术。
