Kozian Alf, Kretzschmar Moritz, Baumgardner James E, Schreiber Jens, Hedenstierna Göran, Larsson Anders, Hachenberg Thomas, Schilling Thomas
Department of Anesthesiology and Intensive Care Medicine, Otto-von-Guericke-University Magdeburg, Germany ; Department of Surgical Sciences, Hedenstierna Laboratory, Uppsala University, Uppsala, Sweden.
Oscillogy ® LLC, Folsom, PA, USA.
Data Brief. 2015 Nov 10;5:939-47. doi: 10.1016/j.dib.2015.11.002. eCollection 2015 Dec.
The data of a corresponding animal experiment demonstrates that nebulized methacholine (MCh) induced severe bronchoconstriction and significant inhomogeneous ventilation and pulmonary perfusion (V̇A/Q̇) distribution in pigs, which is similar to findings in human asthma. The inhalation of MCh induced bronchoconstriction and delayed both uptake and elimination of desflurane (Kretzschmar et al., 2015) [1]. The objective of the present data is to determine V̇A/Q̇ matching by Multiple Inert Gas Elimination Technique (MIGET) in piglets before and during methacholine- (MCh-) induced bronchoconstriction, induced by MCh infusion, and to assess the blood concentration profiles for desflurane (DES) by Micropore Membrane Inlet Mass Spectrometry (MMIMS). Healthy piglets (n=4) under general anesthesia were instrumented with arterial, central venous, and pulmonary artery lines. The airway was secured via median tracheostomy with an endotracheal tube, and animals were mechanically ventilated with intermittent positive pressure ventilation (IPPV) with a FiO2 of 0.4, tidal volume (V T)=10 ml/kg and PEEP of 5cmH2O using an open system. The determination of V.A/Q. was done by MIGET: before desflurane application and at plateau in both healthy state and during MCh infusion. Arterial blood was sampled at 0, 1, 2, 5, 10, 20, and 30 min during wash-in and washout, respectively. Bronchoconstriction was established by MCH infusion aiming at doubling the peak airway pressure, after which wash-in and washout of the anesthetic gas was repeated. Anesthesia gas concentrations were measured by MMIMS. Data were analyzed by ANOVA, paired t-test, and by nonparametric Friedman׳s test and Wilcoxon׳s matched pairs test. We measured airway pressures, pulmonary resistance, and mean paO2 as well as hemodynamic variables in all pigs before desflurane application and at plateau in both healthy state and during methacholine administration by infusion. By MIGET, fractional alveolar ventilation and pulmonary perfusion in relation to the V.A/Q. compartments, data of logSDQ̇ and logSDV̇ (the second moments describing global dispersion, i.e. heterogeneity of distribution) were estimated prior to and after MCh infusion. The uptake and elimination of desflurane was determined by MMIMS.
一项相应动物实验的数据表明，雾化的乙酰甲胆碱（MCh）可诱发猪出现严重的支气管收缩以及显著的通气和肺灌注（V̇A/Q̇）分布不均，这与人类哮喘的表现相似。吸入MCh可诱发支气管收缩，并延迟地氟醚的摄取和清除（Kretzschmar等人，2015年）[1]。本研究数据的目的是通过多惰性气体消除技术（MIGET）测定仔猪在乙酰甲胆碱（MCh）诱发支气管收缩前及期间的V̇A/Q̇匹配情况，MCh诱发支气管收缩是通过静脉输注MCh实现的，并通过微孔膜进样质谱法（MMIMS）评估地氟醚（DES）的血药浓度曲线。对4只处于全身麻醉状态的健康仔猪进行动脉、中心静脉和肺动脉置管。通过经正中气管切开并插入气管内导管确保气道安全，动物采用开放系统进行间歇正压通气（IPPV）机械通气，吸入氧浓度（FiO2）为0.4，潮气量（VT）=10 ml/kg，呼气末正压（PEEP）为5 cmH2O。V̇A/Q̇的测定通过MIGET进行：在地氟醚应用前以及在健康状态和MCh输注期间达到平台期时进行。在洗入和洗出过程中，分别于0、1、2、5、10、20和30分钟采集动脉血样。通过静脉输注MCh使气道峰值压力加倍来诱发支气管收缩，之后重复麻醉气体的洗入和洗出过程。通过MMIMS测量麻醉气体浓度。数据采用方差分析、配对t检验、非参数Friedman检验和Wilcoxon配对检验进行分析。我们在应用地氟醚前以及在健康状态和静脉输注乙酰甲胆碱期间达到平台期时，测量了所有猪的气道压力、肺阻力、平均动脉血氧分压以及血流动力学变量。通过MIGET，测定与V̇A/Q̇分区相关的肺泡通气分数和肺灌注，在MCh输注前后估算logSDQ̇和logSDV̇（描述总体离散度即分布异质性的二阶矩）的数据。通过MMIMS测定地氟醚的摄取和清除情况。
