Boushel R, Langberg H, Olesen J, Nowak M, Simonsen L, Bülow J, Kjaer M
Sports Medicine Research Unit, Copenhagen Muscle Research Centre, Bispebjerg Hospital, Copenhagen, Denmark.
J Appl Physiol (1985). 2000 Nov;89(5):1868-78. doi: 10.1152/jappl.2000.89.5.1868.
Using near-infrared spectroscopy (NIRS) and the tracer indocyanine green (ICG), we quantified blood flow in calf muscle and around the Achilles tendon during plantar flexion (1-9 W). For comparison, blood flow in calf muscle was determined by dye dilution in combination with magnetic resonance imaging measures of muscle volume, and, for the peritendon region, blood flow was measured by (133)Xe washout. From rest to a peak load of 9 W, NIRS-ICG blood flow in calf muscle increased from 2.4+/-0.2 to 74+/-5 ml x 100 ml tissue(-1) x min(-1), similar to that measured by reverse dye (77+/-6 ml x 100 ml tissue(-1) x min(-1)). Achilles peritendon blood flow measured by NIRS-ICG rose with exercise from 2.2+/-0.5 to 15.1+/-0.2 ml x 100 ml(-1) x min(-1), which was similar to that determined by (133)Xe washout (2.0+/-0.6 to 14.6+/-0.3 ml x 100 ml tissue(-1) x min(-1)). This is the first study using NIRS and ICG to quantify regional tissue blood flow during exercise in humans. Due to its high spatial and temporal resolution, the technique may be useful for determining regional blood flow distribution and regulation during exercise in humans.
我们使用近红外光谱技术（NIRS）和示踪剂吲哚菁绿（ICG），对跖屈（1 - 9瓦）过程中小腿肌肉和跟腱周围的血流进行了量化。作为比较，小腿肌肉的血流通过染料稀释结合肌肉体积的磁共振成像测量来确定，而对于肌腱周围区域，血流则通过（133）Xe洗脱来测量。从静息状态到9瓦的峰值负荷，小腿肌肉中NIRS - ICG测量的血流从2.4±0.2增加到74±5毫升×100毫升组织（-1）×分钟（-1），与反向染料测量的结果（77±6毫升×100毫升组织（-1）×分钟（-1））相似。通过NIRS - ICG测量的跟腱周围血流在运动时从2.2±0.5增加到15.1±0.2毫升×100毫升（-1）×分钟（-1），这与通过（133）Xe洗脱确定的结果（2.0±0.6到14.6±0.3毫升×100毫升组织（-1）×分钟（-1））相似。这是第一项使用NIRS和ICG对人体运动期间局部组织血流进行量化的研究。由于其高空间和时间分辨率，该技术可能有助于确定人体运动期间局部血流分布和调节情况。
