Lucero Adam A, Addae Gifty, Lawrence Wayne, Neway Beemnet, Credeur Daniel P, Faulkner James, Rowlands David, Stoner Lee
School of Sport & Exercise, Massey University, Wellington, New Zealand.
School of Public Health, Harvard, Boston, MA, USA.
Exp Physiol. 2018 Jan 1;103(1):90-100. doi: 10.1113/EP086537. Epub 2017 Nov 22.
What is the central question of this study? Continuous-wave near-infrared spectroscopy, coupled with venous and arterial occlusions, offers an economical, non-invasive alternative to measuring skeletal muscle blood flow and oxygen consumption, but its reliability during exercise has not been established. What is the main finding and its importance? Continuous-wave near-infrared spectroscopy devices can reliably assess local skeletal muscle blood flow and oxygen consumption from the vastus lateralis in healthy, physically active adults. The patterns of response exhibited during exercise of varying intensity agree with other published results using similar methodologies, meriting potential applications in clinical diagnosis and therapeutic assessment. Near-infrared spectroscopy (NIRS), coupled with rapid venous and arterial occlusions, can be used for the non-invasive estimation of resting local skeletal muscle blood flow (mBF) and oxygen consumption (mV̇O2), respectively. However, the day-to-day reliability of mBF and mV̇O2 responses to stressors such as incremental dynamic exercise has not been established. The aim of this study was to determine the reliability of NIRS-derived mBF and mV̇O2 responses from incremental dynamic exercise. Measurements of mBF and mV̇O2 were collected in the vastus lateralis of 12 healthy, physically active adults [seven men and five women; 25 (SD 6) years old] during three non-consecutive visits within 10 days. After 10 min rest, participants performed 3 min of rhythmic isotonic knee extension (one extension every 4 s) at 5, 10, 15, 20, 25 and 30% of maximal voluntary contraction (MVC), before four venous occlusions and then two arterial occlusions. The mBF and mV̇O2 increased proportionally with intensity [from 0.55 to 7.68 ml min (100 ml) and from 0.05 to 1.86 ml O min (100 g) , respectively] up to 25% MVC, where they began to plateau at 30% MVC. Moreover, an mBF/mV̇O2 muscle oxygen consumption ratio of ∼5 was consistent for all exercise stages. The intraclass correlation coefficient for mBF indicated high to very high reliability for 10-30% MVC (0.82-0.9). There was very high reliability for mV̇O2 across all exercise stages (intraclass correlation coefficient 0.91-0.96). In conclusion, NIRS can reliably assess muscle blood flow and oxygen consumption responses to low- to moderate-intensity exercise, meriting potential applications in clinical diagnosis and therapeutic assessment.
本研究的核心问题是什么？连续波近红外光谱技术，结合静脉和动脉阻断法，为测量骨骼肌血流量和氧耗提供了一种经济、无创的替代方法，但其在运动期间的可靠性尚未得到证实。主要发现及其重要性是什么？连续波近红外光谱设备能够可靠地评估健康、有体育活动习惯的成年人股外侧肌局部的骨骼肌血流量和氧耗。在不同强度运动期间所表现出的反应模式与使用类似方法发表的其他结果一致，在临床诊断和治疗评估中具有潜在应用价值。近红外光谱技术（NIRS），结合快速静脉和动脉阻断法，可分别用于无创估计静息状态下局部骨骼肌血流量（mBF）和氧耗（mV̇O2）。然而，mBF和mV̇O2对应激源（如递增动态运动）反应的日常可靠性尚未得到证实。本研究的目的是确定NIRS得出的递增动态运动mBF和mV̇O2反应的可靠性。在10天内的三次非连续访视期间，收集了12名健康、有体育活动习惯的成年人（7名男性和5名女性；25岁（标准差6））股外侧肌的mBF和mV̇O2测量值。休息10分钟后，参与者以最大自主收缩（MVC）的5%、10%、15%、20%、25%和30%进行3分钟有节奏的等张膝关节伸展运动（每4秒伸展一次），然后进行四次静脉阻断，接着进行两次动脉阻断。mBF和mV̇O2随强度成比例增加（分别从0.55至7.68毫升·分钟-1·（100毫升）-1和从0.05至1.86毫升·氧·分钟-1·（100克）-1），直至25%MVC，在30%MVC时开始趋于平稳。此外，在所有运动阶段，mBF/mV̇O2肌肉氧耗比约为5保持一致。mBF的组内相关系数表明，在10%-30%MVC时具有高到非常高的可靠性（0.82-0.9）。在所有运动阶段，mV̇O2都具有非常高的可靠性（组内相关系数0.91-0.96）。总之，NIRS能够可靠地评估低至中等强度运动时的肌肉血流量和氧耗反应，在临床诊断和治疗评估中具有潜在应用价值。
