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2
Evidence for interactions between helices 5 and 8 and a role for the interdomain loop in tetracycline resistance mediated by hybrid Tet proteins.
J Biol Chem. 2000 Mar 3;275(9):6101-6. doi: 10.1074/jbc.275.9.6101.
3
The TetA(K) tetracycline/H(+) antiporter from Staphylococcus aureus: mutagenesis and functional analysis of motif C.金黄色葡萄球菌的TetA(K)四环素/H(+)反向转运蛋白：基序C的诱变与功能分析
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Transmembrane remote conformational suppression of the Gly-332 mutation of the Tn10-encoded metal-tetracycline/H+ antiporter.
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Nomenclature for new tetracycline resistance determinants.新型四环素抗性决定簇的命名法。
Antimicrob Agents Chemother. 1999 Jun;43(6):1523-4. doi: 10.1128/AAC.43.6.1523.
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Functional importance and local environments of the cysteines in the tetracycline resistance protein encoded by plasmid pBR322.质粒pBR322编码的四环素抗性蛋白中半胱氨酸的功能重要性及局部环境
J Bacteriol. 1999 Mar;181(5):1689-93. doi: 10.1128/JB.181.5.1689-1693.1999.
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Tertiary contacts of helix V in the lactose permease determined by site-directed chemical cross-linking in situ.通过原位定点化学交联确定的乳糖通透酶中螺旋V的三级接触。
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8
Characterization of Glu126 and Arg144, two residues that are indispensable for substrate binding in the lactose permease of Escherichia coli.对大肠杆菌乳糖通透酶中两个对于底物结合不可或缺的残基Glu126和Arg144的表征。
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Major facilitator superfamily.主要易化子超家族
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Interaction between residues Glu269 (helix VIII) and His322 (helix X) of the lactose permease of Escherichia coli is essential for substrate binding.大肠杆菌乳糖通透酶中位于螺旋 VIII 的谷氨酸 269 残基与位于螺旋 X 的组氨酸 322 残基之间的相互作用对于底物结合至关重要。
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四环素外排蛋白Tet(B)第247位甘氨酸失活替代的第二位点抑制突变

Second-site suppressor mutations of inactivating substitutions at gly247 of the tetracycline efflux protein, Tet(B).

作者信息

Saraceni-Richards C A, Levy S B

机构信息

The Center for Adaptation Genetics and Drug Resistance and the Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts 02111, USA.

出版信息

J Bacteriol. 2000 Nov;182(22):6514-6. doi: 10.1128/JB.182.22.6514-6516.2000.

DOI:10.1128/JB.182.22.6514-6516.2000
PMID:11053399
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC94801/
Abstract

An Asp or Asn substitution for Gly247 in transmembrane helix 8 (TM-8) of Tet(B), the tetracycline efflux protein, eliminated tetracycline resistance. Second site suppressor mutations which partially restored resistance were located in TM-5, -8, -10, or -11 or in cytoplasmic loop 8-9 or loop 10-11. These results indicate physical proximity or functional relationships between TM-8 and these other regions of Tet(B).

摘要

在四环素外排蛋白Tet(B)的跨膜螺旋8（TM-8）中，用天冬氨酸（Asp）或天冬酰胺（Asn）取代甘氨酸247消除了四环素抗性。部分恢复抗性的第二位点抑制突变位于TM-5、-8、-10或-11或胞质环8-9或环10-11中。这些结果表明TM-8与Tet(B)的这些其他区域之间存在物理接近性或功能关系。