四环素外排蛋白Tet(B)第247位甘氨酸失活替代的第二位点抑制突变
Second-site suppressor mutations of inactivating substitutions at gly247 of the tetracycline efflux protein, Tet(B).
作者信息
Saraceni-Richards C A, Levy S B
机构信息
The Center for Adaptation Genetics and Drug Resistance and the Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts 02111, USA.
出版信息
J Bacteriol. 2000 Nov;182(22):6514-6. doi: 10.1128/JB.182.22.6514-6516.2000.
Abstract
An Asp or Asn substitution for Gly247 in transmembrane helix 8 (TM-8) of Tet(B), the tetracycline efflux protein, eliminated tetracycline resistance. Second site suppressor mutations which partially restored resistance were located in TM-5, -8, -10, or -11 or in cytoplasmic loop 8-9 or loop 10-11. These results indicate physical proximity or functional relationships between TM-8 and these other regions of Tet(B).
摘要
在四环素外排蛋白Tet(B)的跨膜螺旋8（TM-8）中，用天冬氨酸（Asp）或天冬酰胺（Asn）取代甘氨酸247消除了四环素抗性。部分恢复抗性的第二位点抑制突变位于TM-5、-8、-10或-11或胞质环8-9或环10-11中。这些结果表明TM-8与Tet(B)的这些其他区域之间存在物理接近性或功能关系。
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