Weinreich S S, Remmerswaal E B, Laport R, van Rhenen D J, Rombout-Sastrienkova E, Smit Sibinga C T, Vrielink H, Boog C J
Department of Immunobiology, Central Laboratory of the Blood Transfusion Service, Amsterdam, The Netherlands.
Vox Sang. 2000;79(2):83-6. doi: 10.1159/000031217.
The beneficial effect of blood transfusion on kidney graft survival requires the presence of leukocytes in the transfusate, but a minimal dose has not been defined, nor has the role of individual leukocyte subsets been investigated. In the Netherlands, a standard pre-transplant blood transfusion consists of a buffy coat (BC)-depleted red blood cell concentrate (RBCC) containing a maximum of 1.2x10(9) residual leukocytes per unit. However, leukocyte subset composition is not standardized.
Using FACS analysis, this study compared the residual leukocyte composition of RBCCs produced by Compomat((R)) and Optipress((R)), two currently used top-bottom systems.
While the total leukocyte content of the RBCCs was equivalent in both press types (0.5x10(9)), the percentage of mononuclear cells (lymphocytes and monocytes) was significantly higher in the Compomat as compared with the Optipress system (p < 0. 0001), resulting in significantly higher numbers of transfused T cells, B cells, HLA-DR-positive cells, NK cells and stem cells.
The leukocyte composition of a pre-transplant blood transfusion depends on the BC depletion method used; this might differentially affect the tolerizing or immunizing potential of a pre-transplant blood transfusion.
输血对肾移植存活的有益作用需要在输注物中存在白细胞，但尚未确定最小剂量，也未研究单个白细胞亚群的作用。在荷兰，标准的移植前输血由去除白细胞层（BC）的红细胞浓缩液（RBCC）组成，每单位最多含1.2×10⁹个残余白细胞。然而，白细胞亚群组成并不标准化。
本研究使用流式细胞术分析，比较了Compomat®和Optipress®这两种目前使用的上下层系统所生产的RBCC的残余白细胞组成。
虽然两种压榨类型的RBCC中白细胞总数相当（0.5×10⁹），但与Optipress系统相比，Compomat系统中单核细胞（淋巴细胞和单核细胞）的百分比显著更高（p < 0.0001），导致输注的T细胞、B细胞、HLA - DR阳性细胞、NK细胞和干细胞数量显著更多。
移植前输血的白细胞组成取决于所使用的去除白细胞层方法；这可能会对移植前输血的免疫耐受或免疫激活潜能产生不同影响。
