Datema G, Stein S, Eijsink C, Mulder A, Claas F H, Doxiadis I I
Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, The Netherlands.
Vox Sang. 2000;79(2):108-11. doi: 10.1159/000031221.
The expression of HLA-C on the surface of platelets is rarely studied due to the lack of proper alloantisera. We addressed this question using an IgM human monoclonal antibody directed against HLA-Cw1 (VP6G3).
Both flow cytometry and complement dependent cytotoxicity studies were used in the current analysis.
The expression of the HLA-Cw1 antigen on platelets is lower than on peripheral blood lymphocytes as shown by flow cytometry. Variation in expression levels between individuals is also observed. Using this antibody in a complement-dependent cytotoxicity assay, we did not observe lysis using platelets as targets, whereas peripheral blood lymphocytes of the same blood donors were adequately lysed.
These results confirm that platelets indeed express HLA-C. Furthermore, the results support the insignificant role of HLA-C in immunological platelet refractoriness.
由于缺乏合适的同种异体抗血清，血小板表面 HLA - C 的表达很少被研究。我们使用一种针对 HLA - Cw1 的 IgM 人单克隆抗体（VP6G3）来解决这个问题。
本分析采用了流式细胞术和补体依赖细胞毒性研究。
流式细胞术显示，血小板上 HLA - Cw1 抗原的表达低于外周血淋巴细胞。个体之间的表达水平也存在差异。在补体依赖细胞毒性试验中使用该抗体时，以血小板为靶标未观察到细胞溶解，而相同献血者的外周血淋巴细胞被充分溶解。
这些结果证实血小板确实表达 HLA - C。此外，结果支持 HLA - C 在免疫性血小板输注无效中作用不显著。
