Edwards J, Grange L L, Wang M, Reyes E
New Mexico Highlands University, Las Vegas, NM 87701, USA.
Phytother Res. 2000 Nov;14(7):517-21. doi: 10.1002/1099-1573(200011)14:7<517::aid-ptr654>3.0.co;2-w.
Of the three flavanolignans that are found in silymarin (Silybum marianum [L.] Gaertn.), silybin is thought to be the primary therapeutic constituent. To test the capacity of silybin to protect the rat fetus from toxic effects of maternally ingested EtOH we did the following: Adult female rats were assigned to one of four groups; EtOH, EtOH/silybin, pair-fed control, and chow fed control. Silybin was orally administered as Siliphos(R), which is one part silybin to two parts phosphatidylcholine. All groups except the chow-fed control were maintained on a liquid diet throughout pregnancy. On day 21 of pregnancy the rats were killed and the fetuses removed. Gamma glutamyl transpeptidase (GGTP) activity and glutathione (GSH) levels were determined for liver and brain tissue for both the fetuses and the dams. Maternal and fetal GGTP activity in the EtOH rats was significantly higher than that of pair-fed controls, whereas the GGTP activity observed in the Siliphos(R)/EtOH rats was not elevated. Fetal mortality rates in the EtOH rats significantly exceeded those of all three other groups.
在水飞蓟宾（水飞蓟 [L.] 盖尔特纳）中发现的三种黄烷木脂素中，水飞蓟宾被认为是主要的治疗成分。为了测试水飞蓟宾保护大鼠胎儿免受母体摄入乙醇毒性影响的能力，我们进行了以下实验：成年雌性大鼠被分为四组之一；乙醇组、乙醇/水飞蓟宾组、配对喂食对照组和普通饲料喂食对照组。水飞蓟宾以Siliphos®的形式口服给药，Siliphos®是一份水飞蓟宾与两份磷脂酰胆碱的混合物。除普通饲料喂食对照组外，所有组在整个孕期都维持液体饮食。在妊娠第21天处死大鼠并取出胎儿。测定了胎儿和母鼠肝脏及脑组织中的γ-谷氨酰转肽酶（GGTP）活性和谷胱甘肽（GSH）水平。乙醇组母鼠和胎儿的GGTP活性显著高于配对喂食对照组，而Siliphos®/乙醇组观察到的GGTP活性并未升高。乙醇组的胎儿死亡率显著超过其他三组。
