Moreland Nicol, La Grange Linda, Montoya Rebecca
Department of Behavioral Sciences, New Mexico Highlands University, 117 Hewett Hall, Las Vegas, NM 87701, USA.
BMC Complement Altern Med. 2002 Nov 11;2:10. doi: 10.1186/1472-6882-2-10.
Using a rat model we have found that the bioflavonoid silymarin (SY) ameliorates some of the negative consequences of in utero exposure to ethanol (EtOH). In the current study our aim was to determine if laterality preference and corpus callosum development were altered in rat offspring whose mothers were provided with a concomitant administration of SY with EtOH throughout gestation.
We provided pregnant Fisher/344 rats with liquid diets containing 35% ethanol derived calories (EDC) throughout the gestational period. A silymarin/phospholipid compound containing 29.8% silybin was co administered with EtOH to a separate experimental group. We tested the offspring for laterality preference at age 12 weeks. After testing the rats were sacrificed and their brains perfused for later corpus callosum extraction.
We observed incomplete development of the splenium in the EtOH-only offspring. Callosal development was complete in all other treatment groups. Rats from the EtOH-only group displayed a left paw preference; whereas control rats were evenly divided between right and left paw preference. Inexplicably both SY groups were largely right paw preferring.
The addition of SY to the EtOH liquid diet did confer some ameliorative effects upon the developing fetal rat brain.
我们利用大鼠模型发现，生物类黄酮水飞蓟素（SY）可改善子宫内暴露于乙醇（EtOH）的一些负面后果。在本研究中，我们的目的是确定在整个妊娠期给母鼠同时给予SY和EtOH的情况下，大鼠后代的偏侧性偏好和胼胝体发育是否会发生改变。
在整个妊娠期，我们给怀孕的Fisher/344大鼠提供含有35%乙醇热量（EDC）的液体饮食。将含有29.8%水飞蓟宾的水飞蓟素/磷脂化合物与EtOH同时给予一个单独的实验组。我们在12周龄时测试后代的偏侧性偏好。测试后，处死大鼠并灌注其大脑以提取胼胝体。
我们观察到仅给予EtOH的后代中胼胝体压部发育不完全。在所有其他治疗组中胼胝体发育是完整的。仅给予EtOH组的大鼠表现出左爪偏好；而对照组大鼠在右爪和左爪偏好之间平均分布。 inexplicably两个SY组大多表现出右爪偏好。
在EtOH液体饮食中添加SY确实对发育中的胎鼠大脑有一些改善作用。
