Warbrick E
Department of Surgery and Molecular Oncology, University of Dundee, Ninewells Hospital and Medical School, Dundee DD1 9SY, UK.
Bioessays. 2000 Nov;22(11):997-1006. doi: 10.1002/1521-1878(200011)22:11<997::AID-BIES6>3.0.CO;2-#.
The identification of proteins that interact with proliferating cell nuclear antigen (PCNA) has recently been a rapidly expanding field of discovery. PCNA is involved in many aspects of DNA replication and processing, forming a sliding platform that can mediate the interaction of proteins with DNA. It is striking that many proteins bind to PCNA through a small region containing a conserved motif; these include proteins involved in cell cycle regulation as well as those involved in DNA processing. Sequential and regulated binding of motif-containing proteins to PCNA may contribute to the ordering of events during DNA replication and repair. Results from bacteriophages and archaea show that the structural basis for the interaction of this motif with PCNA is extremely ancient. The analysis of how such functional motifs have been recruited to proteins in present day organisms helps us to understand how these complex systems arose from ancestral organisms.
鉴定与增殖细胞核抗原（PCNA）相互作用的蛋白质，近来已成为一个迅速发展的发现领域。PCNA参与DNA复制和加工的许多方面，形成一个滑动平台，可介导蛋白质与DNA的相互作用。引人注目的是，许多蛋白质通过包含保守基序的小区域与PCNA结合；这些蛋白质包括参与细胞周期调控的蛋白质以及参与DNA加工的蛋白质。含基序蛋白质与PCNA的顺序性和调控性结合，可能有助于DNA复制和修复过程中事件的有序进行。噬菌体和古细菌的研究结果表明，该基序与PCNA相互作用的结构基础极其古老。分析当今生物体中这些功能基序是如何被招募到蛋白质中的，有助于我们理解这些复杂系统是如何从原始生物体演化而来的。
