Kawai T, Miyama Y, Horiguchi S, Sakamoto K, Zhang Z W, Higashikawa K, Ikeda M
Osaka Occupational Health Service Center, Japan.
Int Arch Occup Environ Health. 2000 Sep;73(7):449-56. doi: 10.1007/s004200000165.
To investigate whether metabolic interactions exist between hexane (HEX) and other solvents when co-exposed at the levels below occupational exposure limits.
Workers, 219 men in ten workshops in total, volunteered to participate in the study. They were occupationally exposed to mixtures of HEX and one or more of toluene (TOL), ethyl acetate (EA) and acetone (ACE). Time-weighted average intensity of vapor exposures was monitored by diffusive personal sampling. 'Free'- and 'total'-2,5-hexanedione (HD) levels in the end-of-shift urine samples were determined by gas chromatography (GC) before and after acid hydrolysis of urine, respectively, and expressed as observed (HDob) or after correction for creatinine concentration (HDcr) or urine specific gravity (HDsg). Possible interaction was examined by multiple regression analysis (MRA), taking either free- or total-HD as a dependent variable, and the four solvent concentrations as independent variables.
In most cases, exposure intensity did not exceed the current occupational exposure limits even when additiveness was assumed. In addition that HEX was the most influential independent variable in all cases as expected, the MRA showed that, in cases of free-HD, ACE was also influential to HDob although weakly, but not to HDcr or HDsg. With regard to total-HD, ACE was weakly influential to HDob and HDsg, and EA also weakly to HDcr. The effect of ACE on free- or total-HD was not detected, however, when 22 men exposed only to HEX and ACE were subjected to the same analysis. Similarly, the effect of EA on total-HD was not observed among the remaining 197 men exposed to HEX, TOL and EA only.
When the exposures were below occupational exposure limits, the free-HD levels in urine after HEX exposure will not be modified by co-exposures to TOL, EA or ACE.
研究在低于职业接触限值的水平下共同暴露时，己烷（HEX）与其他溶剂之间是否存在代谢相互作用。
共有来自十个车间的219名男性工人自愿参与本研究。他们职业性接触HEX与甲苯（TOL）、乙酸乙酯（EA）和丙酮（ACE）中的一种或多种的混合物。通过扩散式个体采样监测蒸气暴露的时间加权平均强度。分别在尿液酸水解前后，采用气相色谱法（GC）测定班末尿样中“游离”和“总”2,5 -己二酮（HD）水平，并分别表示为实测值（HDob）或经肌酐浓度校正后的值（HDcr）或尿比重校正后的值（HDsg）。以游离或总HD为因变量，四种溶剂浓度为自变量，通过多元回归分析（MRA）检验可能存在的相互作用。
在大多数情况下，即使假设存在相加作用，暴露强度也未超过当前职业接触限值。此外，正如预期的那样，HEX在所有情况下都是最具影响力的自变量，MRA显示，对于游离HD，ACE对HDob也有影响，尽管较弱，但对HDcr或HDsg没有影响。对于总HD，ACE对HDob和HDsg有微弱影响，EA对HDcr也有微弱影响。然而，当对仅接触HEX和ACE的22名男性进行相同分析时，未检测到ACE对游离或总HD的影响。同样，在其余仅接触HEX、TOL和EA的197名男性中，未观察到EA对总HD的影响。
当暴露低于职业接触限值时，HEX暴露后尿中游离HD水平不会因同时接触TOL、EA或ACE而改变。
