Schierová M, Linka M, Pazoutová S
Faculty of Sciences, Charles University, Prague 2, Czech Republic.
Curr Genet. 2000 Oct;38(3):126-31. doi: 10.1007/s002940000143.
Two genes for the sulfate assimilation pathway in Aspergillus terreus were cloned. The genes sAT (coding for PAPS-reductase) and sCT (coding for ATP-sulfurylase) form a small gene cluster. Both genes are similar to their homologs in A. nidulans (sA and sC), Penicillium chrysogenum (aps) and Saccharomyces cerevisiae (MET3 and MET16). In the coding sequence of the sCT gene, a typical non-functional APS-kinase-like domain is present. The sCT gene is expressed in A. nidulans, but its expression there is less sensitive to methionine level than in the original species. Two regions 5' upstream of sAT were found to be similar to those of sA.
克隆了土曲霉中参与硫酸同化途径的两个基因。基因sAT（编码PAPS还原酶）和sCT（编码ATP硫酸化酶）形成一个小基因簇。这两个基因与其在构巢曲霉（sA和sC）、产黄青霉（aps）和酿酒酵母（MET3和MET16）中的同源基因相似。在sCT基因的编码序列中，存在一个典型的无功能APS激酶样结构域。sCT基因在构巢曲霉中表达，但其在构巢曲霉中的表达对蛋氨酸水平的敏感性低于在原始物种中的敏感性。发现sAT上游5'端的两个区域与sA的相似。
