Krasnowska M, Medrala W, Małolepszy J, Krasnowski R
Department of Internal Diseases and Allergology, University Medical School, Wrocław, Poland.
Arch Immunol Ther Exp (Warsz). 2000;48(4):287-92.
Interferon gamma (IFN-gamma) is considered one of the causative and intensifying factors in inflammation. The reaction to allergens releases IFN-gamma, an immunomodulatory cytokine known to inhibit IgE synthesis and Th cell proliferation. The aim of the study was to evaluate the influence of IFN-gamma on leukotriene (LT) release in vitro, from human leukocytes of atopic patients with pollinosis and asthma. Thirty-eight patients were enrolled in the study: 15 with pollinosis and 23 asthmatics. In the presence of IL-3, leukocytes were stimulated with specific allergens. Other samples of leukocytes were preincubated with different concentrations of IFN-gamma for 15 min before allergen stimulation. The concentration of LT in supernatants was measured according to the CAST-ELISA procedure. We stated that IFN-gamma had significantly diminished LT release in a dose-dependent mode from the leukocytes of pollinotics. IFN-gamma did not change LT release in the asthmatic group, although, in leukocytes the small and medium basic production of LT, IFN-gamma caused a statistically significant fall in LT generation.
γ干扰素（IFN-γ）被认为是炎症的致病和加剧因素之一。对过敏原的反应会释放IFN-γ，这是一种已知可抑制IgE合成和Th细胞增殖的免疫调节细胞因子。本研究的目的是评估IFN-γ对体外变应性花粉症和哮喘患者白细胞中白三烯（LT）释放的影响。38名患者参与了该研究：15名花粉症患者和23名哮喘患者。在存在IL-3的情况下，用特异性过敏原刺激白细胞。其他白细胞样本在过敏原刺激前用不同浓度的IFN-γ预孵育15分钟。根据CAST-ELISA程序测量上清液中LT的浓度。我们指出，IFN-γ以剂量依赖方式显著减少了花粉症患者白细胞中LT的释放。在哮喘组中，IFN-γ没有改变LT的释放，尽管在白细胞中LT的中小基础产量方面，IFN-γ导致LT生成有统计学意义的下降。
