Escudero R, Barral M, Pérez A, Vitutia M M, García-Pérez A L, Jiménez S, Sellek R E, Anda P
Servicio de Bacteriología, Instituto de Salud Carlos III, 28220 Majadahonda, Madrid, Servicio de Investigación y Mejora Agraria, Departamento de Agricultura, Gobierno Vasco, 48160 Derio, Vizcaya, Spain.
J Clin Microbiol. 2000 Nov;38(11):4026-33. doi: 10.1128/JCM.38.11.4026-4033.2000.
Fifteen Borrelia burgdorferi sensu lato isolates from questing ticks and skin biopsy specimens from erythema migrans patients in three different areas of Spain were characterized. Four different genospecies were found (nine Borrelia garinii, including the two human isolates, three B. burgdorferi sensu stricto, two B. valaisiana, and one B. lusitaniae), showing a diverse spectrum of B. burgdorferi sensu lato species. B. garinii isolates were highly variable in terms of pulsed-field gel electrophoresis pattern and OspA serotype, with four of the seven serotypes described. One of the human isolates was OspA serotype 5, the same found in four of seven tick isolates. The second human isolate was OspA serotype 3, which was not present in ticks from the same area. Seven B. garinii isolates were able to disseminate through the skin of C3H/HeN mice and to cause severe inflammation of joints. One of the two B. valaisiana isolates also caused disease in mice. Only one B. burgdorferi sensu stricto isolate was recovered from the urinary bladder. One isolate each of B. valaisiana and B. lusitaniae were not able to disseminate through the skin of mice or to infect internal organs. In summary, there is substantial diversity in the species and in the pathogenicity of B. burgdorferi sensu lato in areas in northern Spain where Lyme disease is endemic.
对来自西班牙三个不同地区的、从伺机蜱和游走性红斑患者皮肤活检标本中分离出的15株伯氏疏螺旋体狭义种进行了特征分析。发现了四种不同的基因种（9株伽氏疏螺旋体，包括两株人类分离株；3株狭义伯氏疏螺旋体；2株瓦莱西亚疏螺旋体；1株卢西塔尼亚疏螺旋体），显示出伯氏疏螺旋体狭义种的多样谱。伽氏疏螺旋体分离株在脉冲场凝胶电泳图谱和OspA血清型方面高度可变，涵盖了已描述的七种血清型中的四种。其中一株人类分离株为OspA血清型5，在七株蜱分离株中的四株中也发现了相同血清型。第二株人类分离株为OspA血清型3，在同一地区的蜱中未出现。七株伽氏疏螺旋体分离株能够在C3H/HeN小鼠皮肤中播散并引起严重的关节炎症。两株瓦莱西亚疏螺旋体分离株中的一株也能使小鼠致病。仅从膀胱中分离出一株狭义伯氏疏螺旋体。一株瓦莱西亚疏螺旋体和一株卢西塔尼亚疏螺旋体均不能在小鼠皮肤中播散或感染内部器官。总之，在西班牙北部莱姆病流行地区，伯氏疏螺旋体狭义种的种类和致病性存在很大差异。
