Oteo J A, Backenson P B, del Mar Vitutia M, García Moncó J C, Rodríguez I, Escudero R, Anda P
Servicio de Medicina Interna, Hospital de la Rioja, Logroño, Spain.
Res Microbiol. 1998 Jan;149(1):39-46. doi: 10.1016/s0923-2508(97)83622-4.
A skin biopsy from a patient with erythema migrans was inoculated into C3H/He mice and into culture medium. A Borrelia garinii strain named Rio1 was isolated from both a direct BSK medium culture and a mouse ear-punch biopsy culture. Inoculating human tissue into mice produced a disease resulting in severe inflammation of the left tibio-tarsal joint, development of perivascular infiltrates as seen in ear-punch biopsies and the spread of spirochaetes along the skin, far from the inoculation site. The isolation of this strain confirms the circulation of this Borrelia species in Spain as a human pathogen, as well as its arthrogenicity in an animal model. The method used to recover strain Rio1 from human tissue is described as rapid and sensitive compared to direct inoculation of tissue into BSK medium.
将一名游走性红斑患者的皮肤活检样本接种到C3H/He小鼠和培养基中。从直接的BSK培养基培养物和小鼠耳打孔活检培养物中均分离出一种名为Rio1的加氏疏螺旋体菌株。将人体组织接种到小鼠体内引发了一种疾病，导致左胫跗关节严重炎症，出现了耳打孔活检中所见的血管周围浸润，并且螺旋体沿皮肤扩散，远离接种部位。该菌株的分离证实了这种疏螺旋体在西班牙作为人类病原体的传播情况，以及其在动物模型中的致关节炎性。与将组织直接接种到BSK培养基中相比，从人体组织中分离出Rio1菌株的方法被描述为快速且灵敏。
