Lebech A M, Hansen K, Wilske B, Theisen M
Department of Infection-Immunology, Statens Seruminstitut, Copenhagen, Denmark.
Med Microbiol Immunol. 1994 Dec;183(6):325-41. doi: 10.1007/BF00196683.
Twenty-nine European and North American Borrelia burgdorferi strains isolated from patients with Lyme borreliosis, were investigated by restriction fragment length polymorphism (RFLP) of two phylogenetically highly conserved chromosomal genes encoding flagellin (fla) and the p60 common antigen (CA), as well as of the plasmid-borne outer surface protein A (ospA) gene. RFLP of the ospA, fla and CA gene revealed five, two and four distinct subspecies-specific patterns, respectively. RFLP classification of the B. burgdorferi strains was compared with four different classification schemes proposed by others: (i) molecular mass profile of OspA and OspB (Adam et al. [1]); (ii) OspA serotyping (Wilske et al. [34]); (iii) genomic fingerprinting on the central region of the B. burgdorferi fla gene (Picken [24]) and (iv) 16S rRNA signature nucleotide analysis (Marconi and Garon [19]). Results obtained with the different methods correlated highly. All strains classified as B. burgdorferi sensu stricto and B. afzelii could be unequivocally identified as one distinct group by all five typing methods. B. garinii isolates, however, were more heterogeneous and according to RFLP of the CA and ospA gene fell into either two or three subgroups. The agreement of the different approaches supports the recent concept that B. burgdorferi sensu lato strains should be delineated to three genomic groups and that B. burgdorferi sensu lato is clonal. All 12 US strains were B. burgdorferi sensu stricto, whereas the 17 European isolates belonged to any of three genospecies. Among European B. burgdorferi isolates there was an association between B. burgforferi genospecies and the clinical manifestation of Lyme borreliosis. B. afzelii strains were found to predominate in 11 skin isolates (75%), whereas all 6 cerebrospinal fluid isolates from patients with neuroborreliosis were B. garinii. These findings support the concept of a strain-dependent organotropism of B. burgdorferi.
对从莱姆病患者体内分离出的29株欧洲和北美伯氏疏螺旋体菌株，通过对编码鞭毛蛋白（fla）和p60共同抗原（CA）的两个系统发育高度保守的染色体基因以及质粒携带的外膜蛋白A（ospA）基因进行限制性片段长度多态性（RFLP）分析。ospA、fla和CA基因的RFLP分别揭示了5种、2种和4种不同的亚种特异性模式。将伯氏疏螺旋体菌株的RFLP分类与其他人提出的四种不同分类方案进行了比较：（i）OspA和OspB的分子量图谱（Adam等人[1]）；（ii）OspA血清分型（Wilske等人[34]）；（iii）伯氏疏螺旋体fla基因中心区域的基因组指纹图谱（Picken[24]）以及（iv）16S rRNA特征核苷酸分析（Marconi和Garon[19]）。用不同方法获得的结果高度相关。所有被分类为狭义伯氏疏螺旋体和阿氏疏螺旋体的菌株，通过所有五种分型方法都能明确鉴定为一个独特的组。然而，伽氏疏螺旋体分离株更具异质性，根据CA和ospA基因的RFLP分析，可分为两个或三个亚组。不同方法之间的一致性支持了最近的概念，即广义伯氏疏螺旋体菌株应分为三个基因组群，且广义伯氏疏螺旋体是克隆性的。所有12株美国菌株均为狭义伯氏疏螺旋体，而17株欧洲分离株属于三种基因种中的任何一种。在欧洲伯氏疏螺旋体分离株中，伯氏疏螺旋体基因种与莱姆病的临床表现之间存在关联。发现阿氏疏螺旋体菌株在11株皮肤分离株中占主导地位（75%），而所有6株来自神经型莱姆病患者的脑脊液分离株均为伽氏疏螺旋体。这些发现支持了伯氏疏螺旋体菌株依赖性器官嗜性的概念。
