Department of Ophthalmology, Hallym University Dongtan Sacred Heart Hospital, Hallym University College of Medicine, Hwaseong, Korea.
Department of Laboratory Medicine, Hallym University Dongtan Sacred Heart Hospital, Hallym University College of Medicine, Hwaseong, Korea.
Ann Lab Med. 2022 Nov 1;42(6):683-687. doi: 10.3343/alm.2022.42.6.683.
Human adenoviruses (HAdVs) are a major cause of epidemic keratoconjunctivitis. We investigated the types of adenoviruses responsible for the recent epidemic of keratoconjunctivitis in Korea. From January to November 2019, 218 conjunctival swab samples were collected from patients clinically suspected as having adenoviral keratoconjunctivitis. Genotyping targeting of adenovirus capsid hexon genes was performed using PCR and sequencing. Of the 218 samples collected, 128 (58.7%) were positive for the adenovirus genes by PCR, and 126 samples were successfully genotyped. Adenovirus type 8 (HAdV-D8) was the most common type (67.5%), followed by HAdV-D64 (11.1%), HAdV-D37 (9.5%), HAdV-B3 (5.6%), HAdV-D53 (4.0%), HAdV-E4 (1.6%), and HAdV-D56 (0.8%). Adenoviral keratoconjunctivitis cases were the most frequent in July and August 2019, which were mainly caused by type 8. Phylogenetic analyses revealed little genetic distance among adenoviruses of the same type detected in our study. Our results provide basic data for further studies of adenoviral keratoconjunctivitis.
人腺病毒（HAdV）是流行性角结膜炎的主要病因。我们调查了导致韩国近期角结膜炎流行的腺病毒类型。2019 年 1 月至 11 月，从临床上疑似腺病毒性角膜炎的患者中采集了 218 份结膜拭子样本。采用 PCR 和测序方法对腺病毒衣壳 hexon 基因进行了基因分型靶向研究。在采集的 218 个样本中，128 个（58.7%）通过 PCR 检测到腺病毒基因呈阳性，126 个样本成功进行了基因分型。腺病毒 8 型（HAdV-D8）是最常见的类型（67.5%），其次是 HAdV-D64（11.1%）、HAdV-D37（9.5%）、HAdV-B3（5.6%）、HAdV-D53（4.0%）、HAdV-E4（1.6%）和 HAdV-D56（0.8%）。腺病毒性角膜炎病例在 2019 年 7 月和 8 月最为常见，主要由 8 型引起。系统进化分析显示，我们研究中检测到的同一类型的腺病毒之间遗传距离很小。我们的研究结果为进一步研究腺病毒性角膜炎提供了基础数据。
