使用实时PCR杂交分析法检测嗜肺军团菌。
Detection of Legionella pneumophila using a real-time PCR hybridization assay.
作者信息
Ballard A L, Fry N K, Chan L, Surman S B, Lee J V, Harrison T G, Towner K J
机构信息
Department of Microbiology and PHLS Laboratory, University Hospital, Nottingham NG7 2UH, United Kingdom.
出版信息
J Clin Microbiol. 2000 Nov;38(11):4215-8. doi: 10.1128/JCM.38.11.4215-4218.2000.
Abstract
A real-time PCR hybridization assay for Legionella pneumophila is described; the assay uses LightCycler (Idaho Technology) methodology to specifically detect 2.5 CFU/reaction, equivalent to 1,000 CFU/liter of starting water sample. The assay, including DNA extraction and confirmation of product identity, is completed within 90 min of receipt of a sample.
摘要
本文描述了一种用于嗜肺军团菌的实时PCR杂交检测方法；该检测方法采用LightCycler（爱达荷技术公司）方法，能够特异性检测每个反应2.5个菌落形成单位（CFU），相当于每升起始水样中有1000个CFU。该检测方法，包括DNA提取和产物鉴定，在收到样品后的90分钟内完成。
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