Lisby G, Dessau R
Department of Clinical Microbiology, Herlev Hospital, Denmark.
Eur J Clin Microbiol Infect Dis. 1994 Mar;13(3):225-31. doi: 10.1007/BF01974541.
A polymerase chain reaction (PCR) assay for the detection of Legionella spp. in clinical bronchial fluid samples was constructed. The assay could detect a 375 bp fragment of the 16S RNA gene, equivalent to 10 cfu in simulated clinical bronchial fluid and blood samples. Seven clinically relevant Legionella spp. involving 12 serogroups produced a positive signal, whereas three Legionella spp. and none of a panel of 26 bacterial strains produced a signal in the constructed assay. Investigation of bronchial fluid from 51 patients with clinically suspected Legionnaire's disease revealed the presence of Legionella DNA by PCR in seven patients. The results were verified by Southern blot analysis of the amplified DNA fragment. None of 37 control patients produced a positive signal. Only one of the seven bronchial fluid samples positive for Legionella by PCR was positive by conventional culture, thus indicating the increased sensitivity of PCR compared to culture.
构建了一种用于检测临床支气管液样本中军团菌属的聚合酶链反应（PCR）检测方法。该检测方法能够检测到16S RNA基因的一个375 bp片段，在模拟临床支气管液和血液样本中相当于10 cfu。涉及12个血清群的7种临床相关军团菌属产生了阳性信号，而在所构建的检测方法中，3种军团菌属以及26种细菌菌株均未产生信号。对51例临床疑似军团病患者的支气管液进行调查发现，通过PCR检测，7例患者存在军团菌DNA。扩增DNA片段的Southern印迹分析验证了结果。37例对照患者均未产生阳性信号。通过PCR检测为军团菌阳性的7份支气管液样本中，只有1份通过传统培养呈阳性，这表明与培养相比，PCR的灵敏度更高。
