神经肽 α-MSH 和 NPY 调节 RAW 264.7 细胞的吞噬作用和吞噬溶酶体的激活。
The neuropeptides α-MSH and NPY modulate phagocytosis and phagolysosome activation in RAW 264.7 cells.
机构信息
Department of Ophthalmology, Boston University School of Medicine, Boston, MA, USA.
出版信息
J Neuroimmunol. 2013 Jul 15;260(1-2):9-16. doi: 10.1016/j.jneuroim.2013.04.019. Epub 2013 May 17.
Abstract
Within the immunosuppressive ocular microenvironment, there are constitutively present the immunomodulating neuropeptides alpha-melanocyte stimulating hormone (α-MSH) and neuropeptide Y (NPY) that promote suppressor functionality in macrophages. In this study, we examined the possibility that α-MSH and NPY modulate phagocytic activity in macrophages. The macrophages treated with α-MSH and NPY were significantly suppressed in their capacity to phagocytize unopsonized Escherichia coli and Staphylococcus aureus bioparticles, but not antibody-opsonized bioparticles. The neuropeptides significantly suppressed phagolysosome activation, and the FcR-associated generation of reactive oxidative species as well. This suppression corresponds to neuropeptide modulation of macrophage functionality within the ocular microenvironment to suppress the activation of immunogenic inflammation.
摘要
在免疫抑制性的眼部微环境中，存在着固有调节功能的免疫调节神经肽α-黑色素细胞刺激素 (α-MSH) 和神经肽 Y (NPY)，它们促进巨噬细胞的抑制功能。在这项研究中，我们研究了 α-MSH 和 NPY 调节巨噬细胞吞噬作用的可能性。用 α-MSH 和 NPY 处理的巨噬细胞吞噬未经调理的大肠杆菌和金黄色葡萄球菌生物颗粒的能力显著受到抑制，但调理抗体的生物颗粒则不受影响。神经肽显著抑制吞噬溶酶体的激活，以及 FcR 相关的活性氧的产生。这种抑制作用与神经肽在眼部微环境中调节巨噬细胞功能以抑制免疫原性炎症的激活相对应。
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本文引用的文献
1
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2
Endogenous endophthalmitis: 10-year experience at a tertiary referral centre.
Eye (Lond). 2011 Jan;25(1):66-72. doi: 10.1038/eye.2010.145. Epub 2010 Oct 22.
3
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J Neuroimmunol. 2011 Mar;232(1-2):17-25. doi: 10.1016/j.jneuroim.2010.09.025. Epub 2010 Oct 20.
4
The immune privileged retina mediates an alternative activation of J774A.1 cells.
Ocul Immunol Inflamm. 2009 Nov-Dec;17(6):380-9. doi: 10.3109/09273940903118642.
5
Ocular immune privilege.
Eye (Lond). 2009 Oct;23(10):1885-9. doi: 10.1038/eye.2008.382. Epub 2009 Jan 9.
6
Neuropeptide Y promotes TGF-beta1 production in RAW264.7 cells by activating PI3K pathway via Y1 receptor.
Neurosci Bull. 2008 Jun;24(3):155-9. doi: 10.1007/s12264-008-0130-6.
7
Diminishment of alpha-MSH anti-inflammatory activity in MC1r siRNA-transfected RAW264.7 macrophages.
J Leukoc Biol. 2008 Jul;84(1):191-8. doi: 10.1189/jlb.0707463. Epub 2008 Apr 3.
8
Endogenous endophthalmitis: microorganisms, disposition and prognosis.
Acta Ophthalmol Scand. 2007 Dec;85(8):852-6. doi: 10.1111/j.1600-0420.2007.00982.x. Epub 2007 Aug 28.
9
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