Cordoba P, Lanoel A, Grutadauria S, Zapata M
Instituto de Virologia, Facultad de Ciencias Médicas, Universidad Nacional de Cordoba, Cordoba, Argentina.
Clin Diagn Lab Immunol. 2000 Nov;7(6):964-6. doi: 10.1128/CDLI.7.6.964-966.2000.
The protective immune responses against rubella virus (RV) are related to its neutralizing epitopes, an issue that is important to consider when assessing the immune status of patients with remote infection. In the present paper, we compare the antibodies detected by a synthetic-peptide-based enzyme immunoassay (EIA) with antibodies detected by the traditional technique of hemagglutination inhibition (HIA) in patients with remote RV infection. The synthetic peptide used as an antigen (SP15) represents a neutralizing epitope that corresponds to amino acids 208 to 239 of the E1 glycoprotein. The SP15-EIA was developed, all variables that affected the assay were standardized, and the test was validated using reference sera. Serum samples (n = 129) from patients with remote RV infection were tested by HIA and SP15-EIA. Discrepant sera were assayed by MEIA (IMX/Abbot). The comparison between HIA and SP15-EIA, taking HIA as the standard methodology for determining immune status, showed that SP15-EIA is very specific and sensitive for detecting protecting antibodies (specificity, 100%; sensitivity, 98.20%). This study demonstrates that antibodies against the neutralizing domain represented by SP15 would be important in the memory response after natural infection and may be a good tool in the determination of the true immune status of patients with remote infection with regard to RV.
针对风疹病毒（RV）的保护性免疫反应与其中和表位有关，这是评估远期感染患者免疫状态时需要考虑的一个重要问题。在本文中，我们比较了基于合成肽的酶免疫测定法（EIA）和传统血凝抑制技术（HIA）在远期RV感染患者中检测到的抗体。用作抗原的合成肽（SP15）代表一个中和表位，对应于E1糖蛋白的第208至239位氨基酸。我们开发了SP15-EIA，对影响该测定的所有变量进行了标准化，并使用参考血清对该检测方法进行了验证。对远期RV感染患者的血清样本（n = 129）进行了HIA和SP15-EIA检测。对有差异的血清样本采用微粒子酶免疫分析法（MEIA，IMX/雅培）进行检测。以HIA作为确定免疫状态的标准方法，对HIA和SP15-EIA进行比较，结果显示SP15-EIA在检测保护性抗体方面具有很高的特异性和敏感性（特异性为100%；敏感性为98.20%）。本研究表明，针对由SP15代表的中和结构域的抗体在自然感染后的记忆反应中具有重要作用，并且在确定远期RV感染患者的真实免疫状态方面可能是一个很好的工具。
