Zrein M, Joncas J H, Pedneault L, Robillard L, Dwyer R J, Lacroix M
BioChem ImmunoSystems, Montréal, Québec, Canada.
J Clin Microbiol. 1993 Jun;31(6):1521-4. doi: 10.1128/jcm.31.6.1521-1524.1993.
A total of 250 human serum samples were tested for rubella virus immunoglobulin G antibodies by two enzyme immunoassays (EIAs), one using whole rubella virus antigen and the other based on the use of synthetic peptide antigen. The samples were taken from 125 volunteers before and after their immunization with the RA 27/3 rubella vaccine. This study indicates that a synthetic peptide-based EIA can favorably replace current viral lysate-based EIAs to detect rubella virus antibodies following immunization. Because the synthetic peptide used in this newly developed EIA represents a putative neutralization epitope of the rubella virus, it could also be instrumental in determining rubella immune status and in assessing vaccine program efficiency.
采用两种酶免疫测定法（EIA）对总共250份人血清样本进行风疹病毒免疫球蛋白G抗体检测，一种使用完整风疹病毒抗原，另一种基于合成肽抗原。样本取自125名志愿者接种RA 27/3风疹疫苗前后。本研究表明，基于合成肽的EIA能够很好地替代当前基于病毒裂解物的EIA，用于检测免疫后的风疹病毒抗体。由于这种新开发的EIA中使用的合成肽代表风疹病毒的一个假定中和表位，它还可有助于确定风疹免疫状态和评估疫苗接种计划效率。
