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来自酵母线粒体的核糖核酸的信使活性。

Messenger activity of ribonucleic acid form yeast mitochondria.

作者信息

Eggitt M J, Scragg A H

出版信息

Biochem J. 1975 Sep;149(3):507-12. doi: 10.1042/bj1490507.

DOI:10.1042/bj1490507
PMID:1106402
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1165655/
Abstract

Total yeast mitochondrial RNA was shown to possess messenger RNA activity when injected into oocytes of the frog Xenopus laevis. The specific polypeptides formed were precipitated by mitochondrial antisera. A comparison was made of the molecular weights of the proteins obtained form this system with those made by mitochondria in vivo in the presence of cycloheximide. No RNA containing poly(A) sequences was detected in yeast mitochondria.

摘要

当将完整的酵母线粒体RNA注射到非洲爪蟾的卵母细胞中时，它表现出信使RNA活性。所形成的特定多肽能被线粒体抗血清沉淀。将从该系统获得的蛋白质的分子量与在体内存在放线菌酮的情况下由线粒体产生的蛋白质的分子量进行了比较。在酵母线粒体中未检测到含有聚腺苷酸序列的RNA。

相似文献

1
Messenger activity of ribonucleic acid form yeast mitochondria.来自酵母线粒体的核糖核酸的信使活性。
Biochem J. 1975 Sep;149(3):507-12. doi: 10.1042/bj1490507.
2
In vivo uptake of mitochondrial malate dehydrogenase from watermelon by mitochondria of Xenopus laevis oocytes.非洲爪蟾卵母细胞的线粒体对西瓜线粒体苹果酸脱氢酶的体内摄取。
Eur J Cell Biol. 1986 Aug;41(2):263-9.
3
Protein synthesis in an E. coli system directed by yeast mitochondrial RNA.由酵母线粒体RNA指导的大肠杆菌系统中的蛋白质合成。
FEBS Lett. 1976 Jan 1;61(1):6-9. doi: 10.1016/0014-5793(76)80158-5.
4
Synthesis of albumin and malic enzyme in wheat-germ lysates and Xenopus laevis oocytes programmed with chicken-liver messenger RNA.用鸡肝信使核糖核酸编程的小麦胚芽裂解物和非洲爪蟾卵母细胞中白蛋白和苹果酸酶的合成。
Eur J Biochem. 1979 May 2;96(1):1-8. doi: 10.1111/j.1432-1033.1979.tb13006.x.
5
Binding of precursors of cytoplasmically-synthesized mitochondrial proteins to isolated outer membranes of yeast mitochondria.胞质合成的线粒体蛋白前体与酵母线粒体分离的外膜的结合。
Prog Clin Biol Res. 1982;102 Pt B:161-70.
6
Translation of messenger ribonucleic acid from isolated pancreatic islets and human insulinomas.从分离的胰岛和人胰岛素瘤中提取信使核糖核酸的翻译。
Proc Natl Acad Sci U S A. 1975 Dec;72(12):4777-9. doi: 10.1073/pnas.72.12.4777.
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Usage of the three termination codons in a single eukaryotic cell, the Xenopus laevis oocyte.在单个真核细胞——非洲爪蟾卵母细胞中三种终止密码子的使用情况。
Nucleic Acids Res. 1981 Aug 11;9(15):3835-50. doi: 10.1093/nar/9.15.3835.
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Translation of mitochondrial RNA from yeast cytoplasmic petite mutants in an E. coli cell-free system.在大肠杆菌无细胞系统中对酵母细胞质小菌落突变体的线粒体RNA进行翻译。
Biochem Biophys Res Commun. 1975 Jun 16;64(4):1286-92. doi: 10.1016/0006-291x(75)90832-3.
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Translation of RNA that contains polyadenylate from yeast mitochondria in an Escherichia coli ribosomal system.在大肠杆菌核糖体系统中对来自酵母线粒体的含聚腺苷酸的RNA进行翻译。
Proc Natl Acad Sci U S A. 1975 Nov;72(11):4293-7. doi: 10.1073/pnas.72.11.4293.
10
Isolation and identification of yeast messenger ribonucleic acids coding for enolase, glyceraldehyde-3-phosphate dehydrogenase, and phosphoglycerate kinase.编码烯醇酶、3-磷酸甘油醛脱氢酶和磷酸甘油酸激酶的酵母信使核糖核酸的分离与鉴定。
Biochemistry. 1978 Nov 14;17(23):4900-7. doi: 10.1021/bi00616a007.

引用本文的文献

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The synthesis of polyadenylic acid-containing ribonucleic acid by isolated mitochondria from Ehrlich ascites cells.艾氏腹水癌细胞分离线粒体合成含聚腺苷酸的核糖核酸。
Biochem J. 1976 Jun 15;156(3):499-506. doi: 10.1042/bj1560499.

本文引用的文献

1
THE GROWTH AND MAINTENANCE OF TISSUE-CELL CULTURES IN FREE GAS EXCHANGE WITH THE ATMOSPHERE.与大气进行自由气体交换的组织细胞培养物的生长与维持
Am J Hyg. 1963 Sep;78:173-80. doi: 10.1093/oxfordjournals.aje.a120336.
2
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定分子量的可靠性。
J Biol Chem. 1969 Aug 25;244(16):4406-12.
3
A polynucleotide segment rich in adenylic acid in the rapidly-labeled polyribosomal RNA component of mouse sarcoma 180 ascites cells.在小鼠肉瘤180腹水细胞快速标记的多核糖体RNA组分中富含腺苷酸的多核苷酸片段。
Proc Natl Acad Sci U S A. 1971 Jun;68(6):1331-5. doi: 10.1073/pnas.68.6.1331.
4
Proteins coded by mitochondrial DNA of mammalian cells.哺乳动物细胞线粒体DNA编码的蛋白质。
Eur J Biochem. 1971 Dec 10;23(3):564-74. doi: 10.1111/j.1432-1033.1971.tb01655.x.
5
Products of mitochondrial protein synthesis in yeast.酵母中线粒体蛋白质合成的产物。
Nature. 1971 Oct 13;233(5320):196-9.
6
The isolation of polyadenylate with unreacted cellulose.用未反应的纤维素分离聚腺苷酸。
Biochem Biophys Res Commun. 1972 Jun 28;47(6):1426-37. doi: 10.1016/0006-291x(72)90232-x.
7
Use of frog eggs and oocytes for the study of messenger RNA and its translation in living cells.利用蛙卵和卵母细胞研究信使核糖核酸及其在活细胞中的翻译。
Nature. 1971 Sep 17;233(5316):177-82. doi: 10.1038/233177a0.
8
Poly adenylic acid sequences in mitochondrial RNA.线粒体RNA中的聚腺苷酸序列。
Biochem Biophys Res Commun. 1973 Apr 16;51(4):1090-6. doi: 10.1016/0006-291x(73)90039-9.
9
Isolation of eukaryotic messenger RNA on cellulose and its translation in vitro.真核生物信使核糖核酸在纤维素上的分离及其体外翻译
Biochem Biophys Res Commun. 1972 Nov 1;49(3):680-9. doi: 10.1016/0006-291x(72)90465-2.
10
Cytoplasmic type 80 S ribosomes associated with yeast mitochondria. II. Evidence for the association of cytoplasmic ribosomes with the outer mitochondrial membrane in situ.与酵母线粒体相关的胞质型80S核糖体。II. 胞质核糖体与线粒体外膜原位结合的证据。
J Biol Chem. 1974 May 25;249(10):3297-303.