饮用可乐饮料会导致去卵巢大鼠的骨矿化减少。

Cola beverage consumption induces bone mineralization reduction in ovariectomized rats.

作者信息

García-Contreras F, Paniagua R, Avila-Díaz M, Cabrera-Muñoz L, Martínez-Muñiz I, Foyo-Niembro E, Amato D

机构信息

Unidad de Investigación Médica en Enfermedades Nefrológicas, Hospital de Especialidades, México, D.F., Mexico.

出版信息

Arch Med Res. 2000 Jul-Aug;31(4):360-5. doi: 10.1016/s0188-4409(00)00090-4.

DOI:10.1016/s0188-4409(00)00090-4

PMID:11068076

Abstract

BACKGROUND

A significant association of cola beverage consumption and increased risk of bone fractures has been recently reported. The present study was carried out to examine the relationship of cola soft drink intake and bone mineral density in ovariectomized rats.

METHODS

Study 1. Four groups of 10 female Sprague-Dawley rats were studied. Animals from groups II, III, and IV were bilaterally ovariectomized. Animals from groups I and II received tap water for drinking, while animals from groups III and IV each drank a different commercial brand of cola soft drink. After 2 months on these diets, the following were measured: solid diet and liquid consumption; bone mineral density; calcium in bone ashes; femoral cortex width; calcium; phosphate; albumin; creatinine; alkaline phosphatase; 25-OH hydroxyvitamin D, and PTH.

RESULTS

Study 2. Two groups of seven ovariectomized rats were compared. Group A animals received the same management as the group III animals from study 1 (cola soft drink and rat chow ad libitum), while rats from group B received tap water for drinking and pair-feeding. After 2 months plasmatic ionized calcium, phosphate, creatinine, albumin, calcium in femoral ashes, and femoral cortex width were measured. Study 1. Rats consuming cola beverages (groups III and IV) had a threefold higher liquid intake than rats consuming water (groups I and II). Daily solid food intake of rats consuming cola soft drinks was one-half that of rats consuming water. Rats consuming soft drinks developed hypocalcemia and their femoral mineral density measured by DEXA was significantly lower than control animals as follows: group I, 0.20 +/- 0.02; group II, 0.18 +/- 0.01; group III, 0.16 +/- 0.01, and group IV, 0.16 +/- 0.01 g/cm(2). Study 2. To rule out the possibility that these calcium and bone mineral disorders were caused by decreased solid food intake, a pair-fed group was studied. Despite a lower body weight, pair-fed animals consuming tap water did not develop bone mineral reduction or hypocalcemia.

CONCLUSIONS

These data suggest that heavy intake of cola soft drinks has the potential of reducing femoral mineral density.

摘要

背景

最近有报道称可乐饮料消费与骨折风险增加之间存在显著关联。本研究旨在探讨可乐软饮料摄入量与去卵巢大鼠骨矿物质密度之间的关系。

方法

研究1。对四组每组10只雌性斯普拉格-道利大鼠进行研究。第二、三、四组的动物进行双侧卵巢切除。第一组和第二组的动物饮用自来水，而第三组和第四组的动物分别饮用不同商业品牌的可乐软饮料。在这些饮食条件下喂养2个月后，测量以下指标：固体食物和液体摄入量；骨矿物质密度；骨灰中的钙；股骨皮质宽度；钙；磷酸盐；白蛋白；肌酐；碱性磷酸酶；25-羟基维生素D和甲状旁腺激素。

结果

研究2。比较两组每组7只去卵巢大鼠。A组动物的管理方式与研究1中的第三组动物相同（随意饮用可乐软饮料和大鼠饲料），而B组大鼠饮用自来水并进行配对喂养。2个月后，测量血浆离子钙、磷酸盐、肌酐、白蛋白、股骨骨灰中的钙和股骨皮质宽度。研究1。饮用可乐饮料的大鼠（第三组和第四组）的液体摄入量比饮用自来水的大鼠（第一组和第二组）高三倍。饮用可乐软饮料的大鼠的每日固体食物摄入量是饮用自来水的大鼠的一半。饮用软饮料的大鼠出现低钙血症，通过双能X线吸收法测量其股骨矿物质密度显著低于对照动物，具体如下：第一组，0.20±0.02；第二组，0.18±0.01；第三组，0.16±0.01，第四组，0.16±0.01 g/cm²。研究2。为排除这些钙和骨矿物质紊乱是由固体食物摄入量减少引起的可能性，对一个配对喂养组进行了研究。尽管体重较低，但饮用自来水的配对喂养动物并未出现骨矿物质减少或低钙血症。