Kibe Y, Takenaka H, Kishimoto S
Department of Dermatology, Kyoto Prefectural University of Medicine, 465 Kawaramachi-Hirokoji, Kamigyo-ku, Kyoto 602-8566, Japan.
Br J Dermatol. 2000 Oct;143(4):720-7. doi: 10.1046/j.1365-2133.2000.03824.x.
Basic fibroblast growth factor (bFGF) stimulates the mitogenesis of various cells and plays a key part in wound healing.
To determine the spatial and temporal expression of bFGF protein during wound healing after burning of rat skin.
Immunohistochemical methods were used.
The immunostaining for bFGF in the normal epidermis was faint and sporadic in the basal cell layer. However, significant staining for bFGF was found in four locations: regenerated epidermis, a band-like zone near the regenerated epidermis, renewed capillaries, and cells infiltrating into the granulation tissue at the inflammatory to proliferative stages after the burn. The intensity of immunostaining of regenerated epidermis, the band-like zone and renewed capillaries was maximal during the proliferative stage and decreased to normal levels or disappeared simultaneously with wound closure. Immunopositive macrophage-like cell numbers in the granulation tissue increased during the proliferative stage and promptly decreased after wound closure, but such cells were only poorly visible in the scar tissue until 42 days postburn.
bFGF may affect the proliferation, differentiation and migration of regenerated keratinocytes and the recruitment of inflammatory cells, as well as neovascularization in granulation tissue during wound healing. Macrophages may play a pivotal role in cutaneous wound repair by producing bFGF not only during the inflammatory or proliferative stages but also during the remodelling stage.
碱性成纤维细胞生长因子（bFGF）可刺激多种细胞的有丝分裂，在伤口愈合中起关键作用。
确定大鼠皮肤烧伤后伤口愈合过程中bFGF蛋白的时空表达。
采用免疫组织化学方法。
正常表皮中bFGF的免疫染色在基底层微弱且散在。然而，在烧伤后的炎症期至增殖期，在四个部位发现了明显的bFGF染色：再生表皮、再生表皮附近的带状区域、新生毛细血管以及浸润到肉芽组织中的细胞。再生表皮、带状区域和新生毛细血管的免疫染色强度在增殖期最大，随着伤口闭合降至正常水平或消失。肉芽组织中免疫阳性巨噬细胞样细胞数量在增殖期增加，伤口闭合后迅速减少，但在烧伤后42天之前，此类细胞在瘢痕组织中仅隐约可见。
bFGF可能影响伤口愈合过程中再生角质形成细胞的增殖、分化和迁移以及炎症细胞的募集，以及肉芽组织中的新生血管形成。巨噬细胞可能在皮肤伤口修复中起关键作用，不仅在炎症或增殖期，而且在重塑期产生bFGF。
