Malinin T, Ouellette E A
Department of Orthopaedics and Rehabilitation, University of Miami School of Medicine, Miami, Florida 33101, USA.
Osteoarthritis Cartilage. 2000 Nov;8(6):483-91. doi: 10.1053/joca.1999.0324.
To determine the relative importance of subchondral nutrition in cartilage in autologous transplants and its relation to the development of osteoarthritis (OA).
The study was performed in non-human primates with two types of autografts placed orthotopically. One type of autograft was placed into vascularized, viable cancellous bone well, and another in an identical bone well, but coated with methylmethacrylate. The latter prevented direct contact between the autograft and the host bone. Observations were continued for 3 years.
Abrogation of the contact between subchondral bone and articular cartilage-bone autograft had little effect on the cartilage during the first 5-12 months. By 3 years, autografts in the methylmethacrylate wells had non-vascularized and non-viable subchondral bone. The cartilage in these wells underwent degenerative changes compatible with OA.
Interruption of contact between articular cartilage and vascularized subchondral bone resulted in degeneration of the cartilage. The onset and detection of these degenerative changes required long time periods (3 years). Had the experiments been terminated at 1 year or sooner the above described changes would not be apparent.
确定自体移植中软骨下营养在软骨中的相对重要性及其与骨关节炎（OA）发展的关系。
在非人灵长类动物中进行该研究，将两种类型的自体移植物原位植入。一种自体移植物植入血管化的、有活力的松质骨中，另一种植入相同的骨中，但用甲基丙烯酸甲酯包被。后者阻止了自体移植物与宿主骨之间的直接接触。观察持续3年。
在最初的5 - 12个月期间，软骨下骨与关节软骨 - 骨自体移植物之间接触的消除对软骨影响不大。到3年时，甲基丙烯酸甲酯包被的骨中的自体移植物具有无血管化且无活力的软骨下骨。这些骨中的软骨发生了与OA相符的退行性改变。
关节软骨与血管化软骨下骨之间接触的中断导致软骨退变。这些退变改变的发生和检测需要较长时间（3年）。如果实验在1年或更早时终止，上述改变将不明显。
