Huez G, Marbaix G, Hubert E, Cleuter Y, Leclercq M, Chantrenne H, Devos R, Soreq H, Nudel U, Littauer U Z
Eur J Biochem. 1975 Nov 15;59(2):589-92. doi: 10.1111/j.1432-1033.1975.tb02486.x.
Using an ATP:RNA adenyltransferase from Escherichia coli, a polyadenylic sequence was resynthesized onto rabbit globin mRNA from which the poly (A) segment had been previously removed. Conditions for obtaining a homogenous reconstituted globin mRNA preparation containing 30 adenylic residues per message molecule were determined. The reconstituted globin mRNA was microinjected into Xenopus laevis oocytes. Its stability was very similar to that of native mRNA.
利用来自大肠杆菌的ATP：RNA腺苷酸转移酶，在先前已去除多聚（A）片段的兔珠蛋白mRNA上重新合成了多聚腺苷酸序列。确定了获得每个信息分子含有30个腺苷酸残基的均匀重构珠蛋白mRNA制剂的条件。将重构的珠蛋白mRNA显微注射到非洲爪蟾卵母细胞中。其稳定性与天然mRNA非常相似。
