Salvini T F, Belluzzo S S, Selistre de Araújo H S, Souza D H
Laboratório de Neurociências, Departamento de Fisioterapia, Universidade Federal de São Carlos, 13565-905, SP, São Carlos, Brazil.
Toxicon. 2001 May;39(5):641-9. doi: 10.1016/s0041-0101(00)00188-4.
Tibialis anterior (TA) muscles of rats were evaluated 3h, 3 and 30days after intramuscular injection of ACL hemorrhagic toxin I (ACLHT-I, 5mg/kg), partially purified from the venom of Agkistrodon contortrix laticinctus. Contralateral muscles were injected with saline. Three hours after ACLHT-1 injection: presence of hemorrhagic areas and myonecrotic muscle fibers. Three days: injured muscles showed areas in regeneration, some regions with delay of regeneration and bundles of normal fibers. An increased TA muscle weight was found when compared with the contralateral (0.45+/-0.03g versus 0.36+/-0.04g, p=0.04). Thirty days: areas of regenerated muscle fibers presented splits and centralized nuclei. Some regions were replaced by connective tissue. All muscle fiber types were injured but only the incidence of type IIC increased (3.4+/-2.0% versus 0.2+/-0.2%, p=0.0005). Regenerated areas of muscles were exclusively composed by fiber types II and IIC. Regenerated muscles decreased the muscle weight (0.49+/-0.1g versus 0.66+/-0.05g, p=0. 03). In conclusion, ACLHT-I: (a) caused hemorrhage and muscle fiber injury; (b) injured both fiber types I and II; (c) increased the incidence of fiber type IIC and; (d) some muscle regions were replaced by connective tissue.
对大鼠的胫前肌（TA）在肌肉注射从宽带水蝮蛇毒液中部分纯化得到的蝰蛇科出血毒素I（ACLHT-I，5mg/kg）后3小时、3天和30天进行评估。对侧肌肉注射生理盐水。ACLHT-1注射后3小时：出现出血区域和肌坏死肌纤维。3天：受损肌肉显示出再生区域，一些区域再生延迟且有正常纤维束。与对侧相比，TA肌肉重量增加（0.45±0.03g对0.36±0.04g，p = 0.04）。30天：再生肌纤维区域出现分裂和核集中。一些区域被结缔组织取代。所有肌纤维类型均受损，但只有IIC型的发生率增加（3.4±2.0%对0.2±0.2%，p = 0.0005）。肌肉的再生区域仅由II型和IIC型纤维组成。再生肌肉的重量减轻（0.49±0.1g对0.66±0.05g，p = 0.03）。总之，ACLHT-I：（a）导致出血和肌纤维损伤；（b）损伤I型和II型纤维；（c）增加IIC型纤维发生率；（d）一些肌肉区域被结缔组织取代。
