Morris J L, Lindberg C E, Gibbins I L
Department of Anatomy and Histology, Centre for Neuroscience, School of Medicine, Flinders University, G.P.O. Box 2100, S.A. 5001, Adelaide, Australia.
Neurosci Lett. 2000 Nov 24;294(3):167-70. doi: 10.1016/s0304-3940(00)01568-8.
Immunoreactivity (IR) for synaptosomal-associated protein of 25 kDa (SNAP-25) was examined in axons of autonomic vasoconstrictor and vasodilator neurons innervating the lingual and uterine arteries of guinea-pigs. Polyacrylamide gel electrophoresis and immunoblotting of protein extracts demonstrated a SNAP-25-IR band at 25 kDa in both arteries. Quantitative confocal microscopy demonstrated significantly higher levels of SNAP-25-IR in varicosities with IR for vasoactive intestinal peptide (VIP) than in adjacent axons with IR for tyrosine hydroxylase (TH). Levels of SNAP-25-IR in TH-IR axons, relative to adjacent VIP-IR axons, were significantly higher in the lingual artery than the uterine artery. These differences in IR for SNAP-25, a protein considered essential for calcium-dependent exocytosis of neurotransmitters, raise the possibility that mechanisms of transmitter release may vary between different classes of autonomic neurons.
在支配豚鼠舌动脉和子宫动脉的自主缩血管和舒血管神经元的轴突中，检测了25 kDa突触体相关蛋白（SNAP - 25）的免疫反应性（IR）。蛋白质提取物的聚丙烯酰胺凝胶电泳和免疫印迹显示，两条动脉中均有一条25 kDa的SNAP - 25 - IR条带。定量共聚焦显微镜显示，与血管活性肠肽（VIP）呈免疫反应性的曲张体中SNAP - 25 - IR水平显著高于与酪氨酸羟化酶（TH）呈免疫反应性的相邻轴突。相对于相邻的VIP - IR轴突，舌动脉中TH - IR轴突的SNAP - 25 - IR水平显著高于子宫动脉。SNAP - 25是一种被认为对神经递质钙依赖性胞吐作用至关重要的蛋白质，其免疫反应性的这些差异增加了不同类自主神经元之间递质释放机制可能不同的可能性。
