Morris J L, Gibbins I L, Furness J B, Costa M, Murphy R
Neurosci Lett. 1985 Nov 20;62(1):31-7. doi: 10.1016/0304-3940(85)90280-0.
Two major populations of perivascular axons containing immunoreactivity to neuropeptide Y (NPY) have been revealed in the main uterine artery of the guinea pig by immunohistochemical procedures which allow the simultaneous visualization of two antigens. One population contained immunoreactivity to dopamine-beta-hydroxylase (D beta H) and was presumably noradrenergic. The other main population of axons with NPY-like immunoreactivity (NPY-LI) did not have D beta H-like immunoreactivity (D beta H-LI) and was presumably non-noradrenergic. These non-noradrenergic axons also contained immunoreactivity to vasoactive intestinal polypeptide (VIP) and dynorphin (DYN). Indeed, nearly all axons with VIP-LI also contained NPY-LI and DYN-like immunoreactivity (DYN-LI). NPY constricted the uterine artery perfused in vitro, whilst VIP dilated uterine arteries preconstricted with noradrenaline or NPY. Thus, we have evidence for the coexistence of a vasoconstrictor peptide and a vasodilator peptide in the same non-noradrenergic perivascular axons, which also contain an opioid peptide, dynorphin.
通过免疫组织化学方法，在豚鼠子宫主动脉中发现了两个主要的血管周围轴突群，它们对神经肽Y（NPY）具有免疫反应性，该方法可同时显示两种抗原。一群轴突对多巴胺-β-羟化酶（DβH）具有免疫反应性，推测为去甲肾上腺素能。另一主要的具有NPY样免疫反应性（NPY-LI）的轴突群没有DβH样免疫反应性（DβH-LI），推测为非去甲肾上腺素能。这些非去甲肾上腺素能轴突还对血管活性肠肽（VIP）和强啡肽（DYN）具有免疫反应性。实际上，几乎所有具有VIP-LI的轴突也都含有NPY-LI和DYN样免疫反应性（DYN-LI）。NPY使体外灌注的子宫动脉收缩，而VIP使预先用去甲肾上腺素或NPY预收缩的子宫动脉舒张。因此，我们有证据表明，在同一非去甲肾上腺素能血管周围轴突中存在一种血管收缩肽和一种血管舒张肽，这些轴突还含有一种阿片肽——强啡肽。
