Gibbins I L, Morris J L
Department of Anatomy and Histology, School of Medicine, Flinders University of South Australia, Bedford Park.
Brain Res. 1988 Mar 22;444(2):402-6. doi: 10.1016/0006-8993(88)90956-0.
We have used double-labelling immunofluorescence to examine the coexistence of immunoreactivity (IR) to neuropeptide Y (NPY), tyrosine hydroxylase (TH) and vasoactive intestinal peptide (VIP) in autonomic neurons innervating guinea pig cerebral arteries. In the rostral circle of Willis of control animals. NPY-IR was detected in 86% of axons with TH-IR (noradrenergic) and 18% of VIP-IR (non-noradrenergic) axons. No axons contained both VIP-IR and TH-IR. Ten to 12 days after bilateral removal of the superior cervical ganglia all TH-IR axons had disappeared. The density of VIP-IR axons was unchanged but now 70% of VIP-IR axons contained NPY-IR. These results show that NPY is not exclusively associated with noradrenergic axons in the cerebral vasculature. Furthermore, NPY levels in non-noradrenergic axons increased following sympathetic denervation.
我们运用双标免疫荧光法，检测支配豚鼠脑动脉的自主神经元中神经肽Y（NPY）、酪氨酸羟化酶（TH）和血管活性肠肽（VIP）免疫反应性（IR）的共存情况。在对照动物的 Willis 环前部，86%含有 TH-IR（去甲肾上腺素能）的轴突和18%含有 VIP-IR（非去甲肾上腺素能）的轴突中检测到 NPY-IR。没有轴突同时含有 VIP-IR 和 TH-IR。双侧切除颈上神经节后10至12天，所有 TH-IR 轴突消失。VIP-IR 轴突的密度未变，但此时70%的 VIP-IR 轴突含有 NPY-IR。这些结果表明，在脑血管系统中，NPY 并非仅与去甲肾上腺素能轴突相关。此外，交感神经去支配后，非去甲肾上腺素能轴突中的 NPY 水平升高。
